摘要
研究利用喷雾干燥法对革兰氏阴性菌进行微胶囊化处理,以微胶囊产品的菌种存活率和产品的产出率为两个主要考察指标,设计了四因素三水平的正交试验,通过正交试验分析,得出影响微胶囊菌种存活率及产品产出率的优化工艺条件为:进风温度160℃、进料速度12.50ml/min、主要壁材配比试剂a:试剂b为1:9、菌种用量为50ml时,微胶囊中菌种存活率可达84.57%,产出率可达44.62%。微胶囊产品在室温条件下贮存6个月后,活菌数量仍有较大幅度增长,最佳实验条件下菌种增长量为149%。还对微胶囊产品性能进行了表征。
The encapsulation of gram-negative bacteria by spray drying was studied. Two targets, bacterium survival rate and yield of the microcapsule product, were investigated using a four factors and 3D orthogonal test. The optimum condition of spray-drying was achieved, that was 160℃ of inlet air temperature, 12ml/min of feed flow rate, 1：9 of the ratio of GA and AD and 50ml of the liquid growth medium with bacterium. Under above conditions, the bacterium survival rate reached is 84.57% and the yield of the microcapsule product is 44.62%. After the microcapsule product had been reserved in plastic film for 6 months under the environment temperature, the quantity of the viable gram-negative bacteria was increased by 180%.
