垃圾渗滤液的遗传毒性研究

On the hereditary toxicity of the landfilled refuse leachate

应用蚕豆根尖和鲫鱼外周血细胞的微核测定技术对垃圾渗滤液遗传毒性进行了监测。用CODMn为1415mg/L的垃圾渗滤原液处理蚕豆根尖9h,微核千分率（MCN）最高达到（25.62±0.87）‰,同时将垃圾渗滤原液分别稀释到CODMn为710mg/L、470mg/L、280mg/L和200mg/L,在不同时间条件下处理蚕豆根尖,其MCN均呈现出随着处理时间的增长而逐渐增高的趋势;另外,用稀释后CODMn为1.5~14mg/L的垃圾渗滤液喂养鲫鱼,结果也显示,随渗滤液浓度的增高,鲫鱼外周血细胞MCN增大。垃圾渗滤液是一种毒性很强的液体,随着接触垃圾渗滤液的时间增长,会有导致生物体细胞分裂受阻和造成细胞内遗传物质损伤的潜在危害。

The present paper is aimed at introducing its authors＇ study on the hereditary toxic effect of the refuse landfrll leachate. For our purpose, we have done micronucleus tests on the root tip cells of Viciafaba and the peripheral blood red cell of Carassius auratu. Using the organic refuse landfill leachate, we have treated the root tip of Vicia faba for 9 hours and achieved the result of most of MCN reaching （25.62±0.87）‰. At the same time, we have diluted the organic refuse landfill leachate into 710 mg/L, 470 mg/L, 280 mg/L and 200 mg/L by treating the root tip cells of Viciafaba for a different length of time and found that MCN would increase together with the increase of time for treating the cells. On the other hand, when the diluted refuse landfrU leachate （CODMn = 1.5 - 14 mg/L） was used to feed the Carassius auratu, it was found that MCN of the peripheral blood red cell would increase with the increase of certain concentration of the refuse landfill leachate. As the result of such tests of ours, the refuse landfrU leachate has been proved to be a highly-pelluted fluid capable to induce cells to produce micronucleus. Longer period contact with the refuse landfrU leachate would hinder the cell division and induce a potential risk of damage of the internal cytogenetic material within the human bodies.