亚硒酸钠对沙土鼠脑缺血再灌注损伤的海马CA1区神经元超微结构的影响

The Effects of Sodium Selenite on the Ultrastructure of Hippocampus CA_1 of Cerebral Ischemic-reperfused Injury in Gerbils

目的探讨亚硒酸钠对沙土鼠脑缺血再灌注损伤的海马CA1区神经元超微结构的影响。方法采用夹闭双侧颈动脉法制备沙土鼠前脑缺血再灌注模型,双重染色,电镜下观察各组海马CA1区神经元的超微结构变化。TUNEL染色光镜下观察和计数凋亡神经元,计算凋亡密度。结果硒处理组沙土鼠脑缺血再灌注后,神经元超微结构的病理形态损伤减轻,凋亡细胞减少,与对照组比较有显著差异(P<0.01)。结论亚硒酸钠对沙土鼠脑缺血再灌注损伤的海马CA1区神经元超微结构及凋亡细胞具有保护作用,可能存在对脑缺血耐受的机制。

Objective To study the effects of sodium selenite on the ultrastructure of hippocampus CA1 of cerebral Ischemic-reperfused injury in Gerbils. Methods 40 gerbils were randomly divided into three groups： Ⅰ ： sham-operated group; Ⅱ ： cerebral ischemic reperfusion group for 2 days and 4 days; Ⅲ： cerebral ischemic reperfusion for 2 days and 4 days with sodium selenite-treated group. Clip bilateral common carotid arteries in the second and the third groups to make cerebral ischemic reperfusion models. The ultrastructure change of hippocampus was observed by electron microscope. Apoptosis was observed by TUNEL method, the number of apoptosis cells was counted and density was calculated. Results The pathological changes of hippocampus CA1 were few, the number of apoptosis cells was low （ P 〈 0. 001）. Conclusion Sodium selenite could protect neuron against cerebral ischemic reperfusion injury. The mechanisms may be related to cerebral ischemia tolerance.