SPE-HPLC法测定枸杞中甜菜碱的含量

Determination of Glycine betaine in leaf of Lycium Chinese Mill.by SPE-HPLC

目的建立测定枸杞中主要成分甜菜碱含量的方法。方法采用Oasis HLB固相萃取小柱对样品进行纯化，C18色谱柱（5μm，250mm×4．6mm），以50mMKH2PO4（pH4．45）和0．3％辛烷磺酸钠为流动相，流速为0．7mL·min^-1，检测波长200nm。结果甜菜碱进样量在0．01～0．32μg的范围内与峰面积呈良好线性，r=0．9998。结论该法操作简便，结果准确。

OBJECTIVE To establish a method for determination of Glycine betaine in the leaf of Lycium Chinese Mill. by SPE-HPLC. METHODS Seperation was performed on a stainless steel column（5μm,250mm×4. 6mm）,eluted with 50mM KH2PO4 （pH4. 45） containing ion-pair agent 0. 3 % Sodium-1-Octansulfonate at a flow rate of 0. 7mL · min^-1. Detection was performed by UV absorbance at 200nm. RESULTS The calibration curve of Glycine betaine was liner in the range of 0.01～0. 32μg （ r =0. 9996）. CONCLUSION This method was simple,accurate and reliable.