禽胰多肽对肉鸡生长代谢及血浆T_3、T_4的影响

Effects of Avian Pancreatic Polypeptie on Broiler's Metabolization and the Concentrations of T3、T4 in Blood Plasma

禽胰多肽是由禽类动物胰腺的胰多肽细胞分泌的胃肠激素,其分子量为4.2 kD,由36个氨基酸残基组成。为探讨禽胰多肽的生理生化作用,本试验先用磷酸钙吸附法得到了禽胰多肽的粗品,并通过Sephadex-G50层析柱将其纯化,再通过聚丙烯酰胺凝胶电泳进行了鉴定。然后利用自制的禽胰多肽粗品进行动物试验。选择182只AA肉鸡随机分为7组,每组26只,Ⅰ、Ⅱ、Ⅲ、Ⅳ组为饮用组,分别按25、50、75、100 ml/d.组的剂量添加禽胰多肽粗品溶液;Ⅴ、Ⅵ组为注射组,分别按剂量14、28μg/ml.只.周进行皮下注射;Ⅶ组为对照组。在试验开始后的第14、28、42 d,每组中抽取4只鸡采样并进行指标检测。所得数据用SPSS软件分析。结果表明:禽胰多肽粗品能促进鸡的生长发育,改善饲料利用率;能从整体上提高AA肉鸡的代谢水平和免疫力,增强应急能力。

Pancreatic 15olypeptie is a kind of hormone. It secreted from pancreatic polypeptie cell of animal＇s pancreas, and it＇s molecular weight is 4.2 kD, and it is made of 36 amino acids. To study avian pancreatic polypeptie＇s function , crude avian pancreatic polypeptie was acquired by the way of calcium phosphate adsorption. Then it was purified with sephadex-G50 and tested by SDS-PAGE. Afterward, the test on animal is done. 182 broilers were randomly grouped into 7 groups and each group is 26. Ⅰ、Ⅱ、Ⅲ、Ⅳ test group were feed with crude APP by 25,50,75,100 ml/group a day. Ⅴ、Ⅵ test group were injected hypodermically crude APP by 14,28μg/ml a week. Ⅶ test group was control group. Four broilers of each group were killed at 14,28 and 42 days respectively and the swatch were acquired. Then examine the concentrations of TP, RBC, LDH, CK, GOT, GPT, ATP, T3, and T4 in blood plasma or in liver. Analyzing the data by SPSS software showed that crude APP can accelerate broiler＇s growth; ameliorate the feed/gain ratio; advance broiler＇s metabolization and immunity; enhance the feedback while meet an emergency.