制备双特异性单克隆抗体的方法

Methods to Prepare Bispecific Monoclonal Antibodies

双特异性抗体由于其独特的结构-2个不同抗原,引起研究机构的广泛重视。研究者将治疗药物和针对疾病部位的特异目标分别放在双特异性单克隆抗体的2个臂上。治疗药物可以是药品、毒素、酶、DNA和放射性核素等。双特异性单克隆抗体能使免疫效应细胞的细胞毒性作用于致病细胞或者诱导产生全身免疫应答。作者重点介绍了制备双特异性单克隆抗体的3种方法,分别是化学方法、生物方法和基因工程,为制备临床需要的抗体奠定坚实的理论基础。

Bispecific monoclonal antibodies have drawn considerable attention from the research community due to their unique structure against two different antigens. The two-arm structure of BsMAb allows researchers to place a therapeutic agent on one arm while allowing the other to specifically target the disease site. The the- rapeutic agent can be a drug, toxin, enzyme, DNA, radionuclide, etc. Furthermo- re,bsMAb may redirect the cytotoxicity of immune effector cells toward the diseased cell or induce a systemic immune response against the target. This review focuses on therapeutic applications and production of bsMAb while providing the up-to-date clinical trial information.