鸡包涵体肝炎肿瘤坏死因子消长规律的研究

Studies on the Increasing and Decressing of Tumor Necrosis Factor of Chicken Infected with Inclusion Body Hepatitis

自1975年从动物体内提取肿瘤坏死因子(TNF)以来,许多科学家从哺乳动物及禽类体内提取TNF并对其理化性质、生物学活性进行了不同程度的研究和探讨。而用鸡包涵体肝炎病毒诱生TNF与该病之间相关性及产生的消长规律的研究尚属空白。本研究应用鸡包涵体肝炎病毒(FAV-HA毒株)接种SPF雏鸡,在接种后的不同日龄采血,提取TNF,并对其免疫活性(对L929细胞的杀伤率)进行了检测,从而进一步研究其产生TNF的消长规律及与该病毒的相关性。研究结果表明,鸡包涵体肝炎病毒经口腔感染SPF雏鸡可刺激机体产生TNF,在感染的不同日龄鸡的TNF活性出现一定的消长规律。这进一步说明鸡包涵体肝炎(IBH)与TNF的产生及其活性具有密切的相关性。

Many scientists have abstracted the tumor necrosis factor（TNF） from the mammals and avians, and studied its physiological, chemical properties and biological activity at different level, since the first abstraction of the TNF from the animal in 1975. But until now, there are no records of the incressiong and decreasing of TNF and the correlation between the inclusion body hepatitis virus-induced TNF and IBH. The paper was about using chicken inclusion body hepatitis virus to infect SPF chicken and abstract the TNF from the blood and different age and study its immue activity（killing rate to the L929 cells）. We furthermore studied the increasing and decressing of TNF and the correlation between the TNF and virus. The chicken inclusion body hepatitis virus could induce SPF chicken to synthesize TNF with the infection by mouth. Moreover the TNF activities at different age show decreasing and increasing which verity that virus are closely related to the synthesis and activity of TNF.