东海原甲藻对中肋骨条藻的他感作用初探

Allelopathic effects of Prorocentrum donghaiense Lu on Skeletonema costatum

在实验室内将不同生长期的东海原甲藻(Prorocentrum donghaienseLu)培养液滤液用切向超滤技术分级处理后,培养中肋骨条藻(Skeletonema costatum),发现东海原甲藻的滤液对中肋骨条藻的生长具有促进作用,不同生长期的东海原甲藻分泌物在粒级100 ku～0.45μm的培养液中对中肋骨条藻的促进作用最明显,表明东海原甲藻可以产生大小不同的可促进中肋骨条藻生长的他感物质,且在100 ku～0.45μm范围含量较大。东海原甲藻对中肋骨条藻的生长促进作用在不同生长期作用强度不同,促进作用最明显的是消亡期,其次为平台期,指数生长期的藻液是3种藻液中促进作用最小的。

Cross-flow ultrafiltration had been used to isolate the colloidal matter from the cell-free filtrates of Prorocentrum donghaiense Lu in its different growth phases. Monocultures of Skeletonema costatum were exposed to the different size faction retentates and ultrafiltrates of P. donghaiense. The results showed that cell-free filtrates of P. donghaiense positively influenced the growth of S. costatum, and the effects in the retentates of 100 ku-0.45 μm were more intense. The phenomena implied that P. donghaiense could produce the allelopathic substances in various size ranges which could enhance S. costatum＇s growth, but the amounts of the allelopathic substances in range of 100 ku-0. 45μm were higher. The enhanced effects of P. donghaiense on S. costatum were different at different growth phases as follows, the decadency phase〉the stationary phase〉the exponential growth phase.