端粒酶活性检测与非小细胞肺癌的相关性研究

Detection of telomerase activity associated with non-small cell lung cancer study.

目的探讨端粒酶活性在非小细胞肺癌(NSCLC)组织中的表达,以了解其临床意义。方法采用端粒重复序列扩增法一酶联免疫(PCR-TRAP)检测70例肺癌手术患者癌组织中的端粒酶活性,并与70例肺部良性疾病患者对照组的检测结果进行比较。结果端粒酶活性在肺癌组中的阳性率明显高于对照组的阳性率,差异有显著性(P<0.01);Ⅲ期的阳性率明显高于Ⅰ期和Ⅱ期的阳性率,差异有显著性(P<0.05)。结论端粒酶活性检测对NSCLC的诊断具有鉴别意义,有一定的临床辅助价值。

Objective Discuses telomerase activity in the expression of NSCLC, to learn about their clinical significance. Methods Use PCR - TRAP detection of 70 cases of lung cancer patients＇telomerase activity in cancer tissue, and 70 patients with benign lung disease and the results were compared. Results The telomerase activity in the lung cancer group was significantly higher than the positive rate, the difference was significant. 19 was significantly higher than in stages Ⅰ and Ⅱ, the positive rate, the difference was significant. Conclusion Detection of telomerase activity in the diagnosis of NSCLC differentiating, the clinical support a certain value.