摘要
目的:建立对赤阳降脂片中芦丁和槲皮素的含量测定方法。方法:色谱柱:phenomenex Luna C18(150mm×4.6mm,5μm);流动相:甲醇-水-磷酸(47:53:0.2);流速:1mL·min^-1;检测波长:370nm。结果:芦丁在4.8~24.0mg·L^-1范围内线性关系良好;槲皮素在0.48~2.40mg·L^-1范围内线性关系良好,回收率试验结果满意,重复性好。结论:本法简便、准确,可以用于制剂质量控制。
OBJECTIVE To establish an HPLC method for determination of Rutin and quercetin in Chi'yang Jiangzhi tablets. METHODS The column was phenomenex Luna C18 (150 mm× 4, 6 mm, 5μm) the mobile phase consisted of methanol-water-phosphoric acid (47:53:0, 2)at the flow rate of 1 mL·min^-1 and the wavelength was set at 370 nm. RESULTS In this condition there was a good linear relationship over the concentration range of 4, 8 - 24, 0 mg·L^-1 for Rutin, 0. 48 - 2. 40 mg·L^-1 for quercetin. CONCLUSION This method is simple and accurate,it could be applied to the determination of the tables.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2007年第4期490-491,共2页
Chinese Journal of Hospital Pharmacy