益气活血解毒汤对实验性动脉粥样硬化家兔C反应蛋白及血脂水平的影响

Effects of Yiqihuoxuejiedu Decoction on CRP and the Serum Lipid in Rabbits with Experimental Atherosclerosis

目的利用高脂喂饲诱发动脉粥样硬化模型,探讨中药复方益气活血解毒汤对兔动脉粥样硬化的干预作用及其机制。方法24只日本雄性大耳白兔随机分为4组,每组6只,正常对照组、模型对照组、益气活血解毒汤组、辛伐他汀组。其中益气活血解毒汤组及辛伐他汀组边制模边给药,而前两组以等量蒸馏水灌胃。10周后,测定各组空腹血清三酰甘油(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)以及C反应蛋白(CRP)含量。结果模型对照组CRP、TG、TC及LDL-C较正常对照组有不同程度升高,HDL-C下降(P<0.05或P<0.01)。益气活血解毒汤组及辛伐他汀组CRP、TG、TC及LDL-C均明显低于模型对照组,HDL-C升高明显(P<0.01),而与正常对照组比较无统计学意义(P>0.05)。益气活血解毒汤组HDL-C与辛伐他汀组比较有所升高,差异有统计学意义(P<0.05)。结论益气活血解毒汤对动脉粥样硬化有明显的预防和治疗作用。降低CRP和调节血脂可能是其作用机制之一。

Objective The model of atherosclerosis （AS） was established by feeding the cholesterol contained fodder to study the effect of Yiqihuoxuejiedu Decoction on rabbits with atherosclerosis. Methods Twenty-four Japanese white rabbits with large ears were divided randomly into four groups： Control group, model group, Yiqihuoxuejiedu Decoction group and simvastain group. The drugs were administrated along with the replication of the model in Yiqihuoxuejiedu Decoction group and simvastain group, the first two groups intragastric administration by equal quantity of distilled water. After ten weeks, the concentrations of triglyceride （TG）, total cholesterol （TC）, high density lipoprotein cholesterol （HDL - C） and （2 reactive protein （CRP） in each group were measured, Results Compared with the control group, the level of TG, TC, HDL - C and CRP in model group increased and HDL - C decreased （P 〈 0.05 or P 〈 0.01 ）, Compared with the model group, the concentrations of TG, TC, LDL- C and CRP in Yiqihuoxuejiedu decoction group and Simvastain group decreased obviously and HDL - C increased （ P 〈 0.01 ）, But there was no significant difference compared with the control group （ P 〉0.05）, Compared with the Simvastain group, level of HDL- C increased in Yiqihuoxuejiedu decoction group （P 〈 0, 05）. Conclusion Yiqihuoxuejiedu decoction was a potential drug for treatment and prevention of atherosclerosis. The effect of Yiqihuoxuejiedu decoction depended most probably on the decrease of CRP and regulation of the serum lipid.