糖耐量受损大鼠血清脂联素和TNF-α与冠状动脉病变的关系探讨

The Relationship between Adiponectin,TNF-α and Coronary Artery Lesion in IGT Rats

目的探讨血清脂联素与肿瘤坏死因子-α(TNF-α)在糖耐量受损(IGT)期的变化及其与大鼠冠状动脉病变的关系。方法将Wistar大鼠随机分为对照组和IGT组,采用高脂饮食诱导糖耐量受损模型采用酶联免疫法、放免法检测血清脂联素、TNF-α等指标,在光镜下观察冠状动脉的病理变化并测定冠状动脉内中膜的厚度。结果IGT组大鼠血清脂联素在治疗8周时较正常对照组下降(P<0.05),TNF-α治疗12周时较正常对照组显著上升(P<0.01);左冠状动脉内中膜厚度在治疗12周、20周时均较正常对照组明显增加(P<0.01)。结论血清脂联素下降、TNF-α升高与IGT期冠状动脉病变发生显著相关,提示血清低脂联素、高TNF-α参与了心血管病的发生。

Objective To investigate the role of adiponectin and TNF- α in the development of coronary artery lesion in IGT rats. Methods Wistar rats were randomly divided into normal control group and IGT group. The early pathological changes of the coronary artery were examined using light microscope and the coronary anatomy intima - media thickness was measured. Plasma concentration of adiponectin and TNF- α were measured by ELISA and RIA. Results The early stages of coronary artery lesion occurred in IGT 12 weeks. The decrease of plasma adiponectin were observed at 8 weeks while the increase of plasma TNF - α was observed at 12 week. Compared with normal control group, the coronary artery intima-media thickness was increased markedly at 8 and 12 weeks. Conclusion The decrease of adiponectin occurred before visible and measurable coronary artery pathological changes. In the progression of IGT coronary artery lesion, adiponectin might play an important role. TNF- α was harmful. It could be used as an early figure to predict IGT coronary artery lesion.