连续式酶反应器中脂肪酶催化合成麦芽糖月桂酸酯的研究

Immobilized Lipase-Catalyzed Synthesis of Lauroyl Maltose Using a Continuous Stirred Tank Reactor

利用连续式酶反应器(CSTR)、固定化脂肪酶催化合成麦芽糖月桂酸酯。确定了连续生产工艺的最佳条件:在丙酮介质中,起始反应器中麦芽糖的浓度为50 mmol/L,月桂酸浓度为200mmol/L,加酶量为10 g,每天添加4 g麦芽糖,200 mmol/L的月桂酸丙酮溶液以0.15 mL/min进入反应器。本反应器的麦芽糖酯产量可以达到12.32 g/(L.d),麦芽糖的转化率为58%,高于间歇式生产,而且在生产中不需要添加分子筛。研究了产物的分离纯化方法,利用正己烷对产物进行3次洗涤、离心处理,得到的产物麦芽糖月桂酸酯纯度达到94.16%。

In this manuscript, lauroyl maltose was synthesized using a continuous stirred tank reactor through the immobilized lipase-catalyzed condensation of lauric acid and maltose. The 1 optimal condition for continuous production in acetone is as follows： 50 mmol/L maltose, 200 mmol/L lauric acid and 10 g immobilized lipase were added to the reactor at the initral of the operation, 4 g/day of maltose was added, lauric acid dissolved in acetone at a concentration of 200 mmol/L was fed to the reactor at a flow rate of 0. 15mL/min. The productivity of this reactor could be reach at 12.32 g/（L· d） ,which higher that that of the batch reaction. The conversion of maltose is 58% ,and without molecular sieves included. The separation and purification of lauroyl maltose was also studied. When the reaction mixture being deacidified with hexnal followed by centrifugation for three times, the final product was purified to he 94.16% of lauroyl maltose.