应用斑点金免疫渗滤试验测定阴道加特纳菌抗体

The Application of DIGFA in the Detecting of Gardnerella Vaginalis Antibody

目的探讨金免疫测定技术在免疫学检验的临床应用,建立阴道加特纳菌抗体检测的方法。方法用斑点金免疫渗滤试验。结果阴道分泌物涂片找线索细胞阳性率为21%,此法对加特纳菌抗体阳性检出率为38%;χ2检验P<0.01。结论此法快捷简单,可间接测定加特纳菌的感染。

Objective To explore the clinical application of immunogoldtechnlc,on the basis to find a method of detecting the Gardnerella vaginalis （GV） antibody. Methods 55 cases of bacterial vaginosis by dot immunogold filtration assay and with compared the way of searching the clue cells of vaginal secretion stain. Results In the way of searching the clue cells of vaginal secretion stain,the positive rate was 21% ,and the detective rate of the Gardnerella vaginalis antibody by DIGFA was 38%. Through χ^2 test,P was under 0. 01. Conclusion This rapid and simple way can be adopted to indirectly detect the infection of GV.