SELDI-TOF-MS技术筛选胃癌血清标志物研究

Study on Serum Biomarkers Screening for Gastric Cancer Using SELDI-TOF-MS Technology

[目的]筛选胃癌患者血清肿瘤标志物,用于胃癌早期诊断。[方法]应用表面增强激光解析离子化飞行时间质谱技术(Surface Enhanced Laser Desorption/Ionization Time-of-flight Mass Spectrometry,SELDI-TOF-MS),检测32例胃腺癌、22例消化性溃疡、32例胃炎患者和30名正常人(对照)的外周血清标本,筛选胃癌相关特异蛋白峰。[结果]与正常对照相比较,发现质荷比为2953、3267、5341、5912、5927的蛋白质分子在胃癌患者血清中上调,质荷比为4059、4213、4270、7160的蛋白质在胃癌患者中下调。4059、5341、5912、3267、4059及5341的蛋白质组成的波谱模型可以准确鉴别胃癌与正常对照。其中质荷比为5912的蛋白质在胃癌患者血清中有很高的表达,敏感性为81．25％,特异性为56．67％。4059的波谱峰可以在正常组中显示,但在胃癌组中几乎消失。[结论]SELDI-TOF-MS技术是一种快速、简便、易行且高通量的分析方法。本研究筛选出的4059、5341、5912、3267、4059及5341低分子量蛋白质有可能成为潜在的诊断胃癌的血清生物标志物。

[ Objective ] To discover new serum biomarkers of gastric cancer for early detection. [ Methods ] Surfaceenhanced laser desorption/ionization time-of-flight mass spectroscopy（ SELDI-TOF-MS ）was used to generate proteomic spectra of 32 patients diagnosed as gastric adenocareinoma, 22 patients as peptic ulcer, 32 patients as gastritis, and 30 normal persons. Then the spectra were analyzed with Ciphergen Biosystem＇s Software and a new Biomarker Pattern＇s Software. [ Results ] Five masses with an average mass of 2 953, 3 267, 5 341, 5 912, 5 927 were up-regulated in gastric cancer compared with normal persons, and four masses of 4059, 4213, 4270, 7 160 were down-regulated. In these masses, 4213, 5341, 5912, 7 160 masses have higher mean peak intensity. These 4 masses also changed significantly in PU and GS. Peak 5912 is the most interesting protein with a sensitivity of 81.25%, and specificity of 56.67%. Peak 4059 is a small but consistent peak which can be clearly seen from control samples but disappears in patient samples. Combination of three markers （ 4 059, 5 341, 5 912 ） or （ 3 267, 4 059, 5 341 ） could distinguish effectively gastric cancer with normal persons. [ Conclusion ] These low molecular weight serum proteins may be hopeful biomarkers which can be help to diagnose gastric cancer.