热化疗对肺部肿瘤细胞生长影响的研究

A Study on the Effect of Thermotherapy with Chemotherapy on Lung Tumor Cell Growth

[目的]研究热化疗联合对肺部肿瘤细胞生长的影响。[方法]构建人谷胱甘肽硫转移酶P1(glutathione- s-transferase P1,GSTP1)基因的真核表达载体,转染人肺腺癌A549细胞,然后按临床常用剂量,用6．25、12．50、25．00、50．00、100．00、200．00μg/L紫杉醇处理转染和未转染的A549细胞,并联合43℃加热,用噻唑蓝比色法(MTT法)和流式细胞术(FCM)检测转染前后A549细胞在不同条件下的生长和细胞周期分布,并运用SPSS 11．5对数据进行统计分析。[结果]两种细胞在6．25、12．50、25．00、50．00、100．00μg/L浓度的紫杉醇作用下,未转染细胞增殖率低于转染细胞(P< 0．05)。43℃加热,两种细胞在6．25、12．50、25．00、50．00、100．00、200．00μg/L浓度的紫杉醇作用下,未转染细胞增殖率高于转染细胞(P<0．05);流式细胞仪检测两种细胞的生长周期,与未转染细胞相比,转染细胞的G0／G1期(53．30％与67．40％)和G2M期(8．82％与19．60％)延长,S期缩短(37．90％与13．00％),均P<0．05。[结论]热化疗联合治疗可以逆转肺部肿瘤细胞的耐药,为进一步研究预防肺癌发生及其与职业性肺癌的关系奠定了基础。

[ Objective ] To study the effect of thermotherapy with chemotherapy on lung tumor cell growth. [ Methods ] A eukaryotic expression vector carrying human glutathione-s-transferase P1 gene was constructed and transfected into A549 cells and then the cells were treated with different dosage of paclitaxel. MTT assay and FCM were used to measure survival and cell cycle. Cells without GSTP1 gene were used as controls. [ Results ] The proliferation rate of cells without GSTP1 was lower than that of cells with GSTP1 at 6.25.12.50.25.00.50.00. 100.00μg/L of paclitaxel （ P 〈 0.05 ）, whereas under the heating, the proliferation rate of cells without GSTP1 was higher than that of cells with GSTP1 at 6.25, 12.50. 25.00.50.00. 100.00. 200.00μg/L of paclitaxel （ P 〈 0.05 ）. FCM also showed Go/G, （ 53.30% Vs 67.40% ） and G2M （ 8.82% Vs 19.60% ） phase of cells with GSTP1 became longer and S phase（ 37.90% Vs 13.00% ）was shorter, P 〈 0.05. [ Conclusion ] Thermotherapy with chemotherapy can reverse the drug-endurance of lung tumor cells. This phenomenon may be helpful in the further research on the prevention of lung cancer and its relationship with occupation.