禽致病菌ESBLs和AmpC酶的检测及药敏分析

Detection of Extended-spectrum β-lactamases and AmpC Enzyme and Antibiotics Susceptibility Test Analysis of Pathogens Isolated from Poultry

用全自动微生物鉴定系统(VITEK-32)鉴定了禽分离致病菌,分别进行了β-内酰胺酶(BLA)、超广谱β-内酰胺酶(ESBLs)、AmpC酶的检测,并用试管两倍稀释法测定了各种抗生素对非产酶菌、产ESBLs菌及产AmpC菌的抗菌活性。结果表明,鉴定分离的20株致病菌有大肠埃希菌15株、阴沟肠杆菌1株、铜绿假单胞菌1株、法氏柠檬酸杆菌1株、肺炎克雷伯菌1株及鹑鸡肠球菌1株,其中法氏柠檬酸杆菌、肺炎克雷伯菌及鹑鸡肠球菌系兽医上首次检出。报道所分离的20株致病菌均产β-内酰胺酶,其中产ESBLs 9株,同时产ESBLs和AmpC酶1株。产酶菌株对抗生素的耐药性严重,而抗生素/抑制剂联用能降低药物对细菌的MICs。

The antibacterial activity of various antibiotics or beta-lactams with inhibitors against non-beta-lactamase-prouding, extended-spectrum beta-lactamase（ESBLs）-producing and AmpC enzyme producing isolates were evaluated with two-fold dilution method after pathogens isolated from poultry were identificated with VITEK-32 and were detected respectively for beta-lactamase,ESBLs and AmpC enzyme. The results revealed that there are 15 Escheria coli , l Enterobacter cloacae, 1 Pseudomonas aeriginosa,1 Citrobater farmeri, 1 Klebsiella pneumoniae and 1 Entercoccus gallinarum in the 20 pathogens. In these pathogens, the citrobacter farmeri, Klebsiella pneumoniae and Enterococcus gallinarum were first isolated from poultry in veterinary medicine. All of the isolated strains could produce beta-lac- tamase,8 strains could produce ESBLs and 1 strains could produce ESBLs and AmpC enzyme. All of the 20 strains isolated were resistant to antibiotics except for the imipenem and meropenem. The combination of beta-lactams with inhibitors could decrease the MICs of antibiotics against isolates.