大气自然降尘与单株人体正常菌的体外相互作用研究

Study on the reciprocity between atmosphere natural dropping dusts and single strain human body surface normal bacteria in vitro

对5种大气自然降尘主要化学成分进行分析,并对其与表皮葡萄球菌相互作用后各培养液中pH值、葡萄糖(GLU)、电解质以及Mn2+、Zn2+、Fe3+、Ni3+等浓度的变化进行测定,以比较粉尘的化学成分及物理性状对试验目标菌生长的影响.同时通过SEM分析对粉尘与细菌作用过程中细菌形态和界面作用的情况进行了研究.结果表明,各种大气尘粒所含化学成分不同,与细菌作用后离子溶出量也不完全相同;Ca2+或Mg2+含量高的大气尘粒能使表皮葡萄球菌数量增大并增加其对GLU的利用;粉尘颗粒越小,越易与菌体结合;另外,粉尘颗粒形状的不规则性也大大增加了其与菌体间的膜界结合程度.

In order to compare the chemical compositions and physical properities of dusts on S. epidernidis, chemical compositions of five kinds of atmosphere natural dropping dusts have been analyzed and the changes of pH value, glucose （GLU）, electrolyte and Mn^2 ＋ , Zn^2 ＋ , Fe^3 ＋ , Ni^3 ＋ before and after the dusts reacted with S. epidernidis have been measured. The SEM analysis has been used to study the bacterial form and interface action status in the course of reaction between dusts and bacteria. The results illustrated that Major chemical composition of atmosphere dusts and dissolution quantity of ions after the aced with S. epidernidis were different. The atmosphere dusts containing high Ca^2 ＋ or Mg^2 ＋ could increase the number of S. epldernidis and the use quantity of GLU. The dusts granule is more small, more easy adhere to thalli, and the abnormity of shape also can increase the adhesion degree of filminterface between dusts and bacteria.