利用细胞工程建立的耐低磷玉米自交系与原自交系耐低磷性状的比较

Comparisons Between Low-phosphorus Tolerance Maize Inbred Lines Established by Cell Engineering and Origin Inbred Line

以细胞工程技术获得的耐低磷玉米自交系99043、99038及玉米骨干自交系齐319为材料,利用砂培方法,探讨耐低磷自交系生理特性的差异,初步筛选磷高效育种材料。将自交系99043、99038和齐319播在装有黄砂的塑料盆中进行耐低磷特性的测定。自交系99043与齐319相比表现出明显的耐低磷特性,即叶片出现缺磷症状的时间延迟,发生50%植株死亡所需的时间增长。

Physiological characteristics of the different low- phosphorus tolerance maize inbred lines were probed under sand culture conditions, these low - phosphorus tolerance maize inbred lines include 99043 and 99038 acquired by cell engineering technology and the skeleton maize inbred line Qi319. The primary purpose of these studies is to elucidated the possible mechanisms of the low - phosphorus tolerance maize lines and cultivate Pi high efficiency germplasm. In the sand culture experiments, 99043 and Qi319 were grown in the plastic pots for 35d, The seedlings were watered with high （ ＋ P, 1000uM Pi） and low （ - P, Pi deprived of） Pi nutrient solutions every day. The results indicated that the differences in the absorption, transition and utilization efficiency of P between Qi319 and 99043/99038 were obvious.