Genistein对鼻咽癌细胞增殖抑制作用的研究

Inhibition of Genistein on proliferation of CNE cells in nasopharyngeal cancer

目的:探讨酪氨酸蛋白激酶(TPK)抑制剂Genistein对鼻咽癌细胞增殖的抑制作用。方法:以不同浓度的Genistein作用于鼻咽癌细胞,采用MTT法检测细胞的增殖程度,倒置显微镜下观察肿瘤细胞的形态学变化。结果:Genistein引起鼻咽癌细胞增殖比明显下降,其程度随Genistein浓度增高而增强;Genistein促进鼻咽癌细胞形态发生改变并导致细胞死亡。结论:Genistein对鼻咽癌细胞的增殖有抑制作用,并破坏肿瘤细胞的结构而使其死亡。

Objective： To investigate the inhibition of Genistein on the proliferation of nasopharyngeal cancer cells. Methods： Cultured CNE cells were stimulated with different doses of Genistein. The proliferation of CNE cells were determined by MTT, the morphological changes of tumor cell were observed under inverted microscope respectively. Results： Proliferation ratio of CNE cells was significantly decreased by Geinstein in dose dependent manner and it promoted cancer cellular form changes as well as causesed the cell death. Conclusion： Genistein could inhibit the proliferation and destroy the tumor cell shape and cause death.