用于准确评价酵母絮凝性能的新方法研究

A Novel Method for Accurate Measurement of Yeast Flocculence

建立了1种快速、灵敏分析特定酵母絮凝性能的新方法。通过考察酵母悬浮液初始浓度、缓冲液pH值、各组分用量比例、体系水质等多因素对酵母絮凝性能的影响规律,优化确立了该测定体系的最佳参数:pH3.90的缓冲液2.7 mL和0.8 mL初始浓度0.3 g/mL的酵母悬浮液,体系适宜水质选择经离子交换处理后的纯净水。该方法操作简便,测定结果准确可靠。

This paper introduced a novel method for determining flocculence of yeast cell. Through investing effects of such factors as concentration of yeast suspension, pH value of buffer, ratio of components, and type of water quality on yeast flocculation, the optimal conditions were obtained as following： 0.3 g/mL yeast suspension, 2.7 mL buffer with pH ：3.90, and purified water by ion exchange treatment, respectively. The optimized method was characteristic of simpleness and sensitiveness.