癌痛消颗粒影响大鼠移植性肝癌细胞凋亡的研究

The Effect of Aitongxiao Granule on Apoptosis in Liver Implanted Walker-256 Carcinosarcoma

目的:探讨癌痛消颗粒对实验大鼠肝癌细胞的影响。方法:采用Walker-256瘤株进行SD大鼠的移植型肝癌的造模,造模结束后药物治疗,最后处死动物取标本,检测细胞周期及细胞凋亡。结果:癌痛消颗粒组的肝细胞主要停留在细胞周期的G0/G1期,在S期的细胞明显减少,与模型组相比,差异均有非常显著性意义(P<0.01);癌痛消组细胞凋亡率明显提高,与模型组、正常组比较,差异均有非常显著性意义(P<0.01)。结论:癌痛消颗粒能使肝癌细胞周期停留在G0/G1期,阻止其进入S期;显著提高癌细胞凋亡率,促使癌细胞凋亡。

Objective： To explore the effect of Aitongxiao granule（ATX） on Spradue - Dawley rats which were implanted liver tumors（Walker-256 carcinosarcoma）. Methods： 30 spradue- Dawley rats were implanted Walker- 256 carcinosarcoma in their livers, and were divided into 3 groups randomly. After the model was made, rats in 3 groups were treated with aqua stillata, 5 - Fu and ATX respectivelyl After treatment, the animals werc killed and their transplanted tumors were cut off to be detected generation cycle and apoptosis in liver tumor cells. Results： The bulk of cells of liver tumors in group ATX stayed in G0/G1 phase. Cells in S phase were markedly decreased. Compared to that of the model group, there were significant differences altogether（ all P 〈 0. 01 ）. The apoptosis rates of tumor cells in every group ATX were obviously elevated, compared with that of the model group and the normal group, there were significant differences altogether（ all P 〈 0. 01 ）. Conclusion： ATX could retain cells of liver tumors staying in G0/G1 phase and prevent the Cells from Gl phase to S phase. It could also markedly improve apoptosis rates of tumor cells, and induce apoptosis of liver tumor cells.