葛花醒酒养胃颗粒对酒精性肝病内毒素损伤的防治作用

Formulation of mechanism and experimental study of Gehua Xingjiu Yangwei granule (GHXJYWG) against endotoxin injury in rats alcoholic liver disease

目的:探讨葛花醒酒养胃颗粒对酒精性肝病(ALD)内毒素损伤的防治作用。方法:Wistar大鼠48只随机分为正常组、模型组、葛花醒酒养胃颗粒治疗组(简称治疗组)和肝泰乐对照组(简称对照组)。实验采用梯度酒精定量灌胃法制备酒精性肝病大鼠模型,造模同时治疗组予葛花醒酒养胃颗粒汤150mg.kg-1.d-1,分2次灌胃,对照组予肝泰乐水38mg.kg-1.d-1,分2次灌胃,正常组予同容积生理盐水,每日灌胃2次,连续灌胃8周后采血,检测大鼠血浆内毒素(LPS)、血清酶(ALT、AST、GGT)、血脂(Tch、TG)及血清肿瘤坏死因子(TNF-α)的含量,同时用RT-PCR法测定肝组织中脂多糖结合蛋白(LBP)和脂多糖受体CD14mRNA的表达。结果:模型组大鼠血浆内毒素和血清TNF-α水平明显高于对照组(P<0.05),肝组织中LBP和CD14mRNA的表达明显高于对照组(P<0.05);同时血清ALT、AST、GGT、TG明显升高(P<0.05);治疗组血浆内毒素和血清TNF-α水平明显低于模型组(P<0.05),LBP和CD14mRNA的表达明显低于对照组(P<0.05),血清ALT、AST、GGT、TG水平明显降低(P<0.05)。结论:葛花醒酒养胃颗粒对大鼠酒精性肝损伤有较好的防治作用。

Objective： To discuss action of Gabua Xingsiu yangwei gramule GHXJYWG against endotoxin injury in rats alcoholic liver disease. Methods： Forty-eight Wistar rats were divided into four groups ： normal, Model, treatment with GHXJYWG, comparison with glucurone, model was filled with alcohol, the group of GHXJYWG was gastric infusion with GHXJYWG liquor （150mg · kg^-1· d^-1）, the group of contrast was gastric infusion with glueurone water （38mg · kg^-1· d^-1）, the norreal was filled with normal saline, the rats was killed at the end of gastrieing infusion consecutive 8 week, to measure plasma endotoxin, blood serum enzyme （ALT, AST, GGT）, blood fat （TCh, TG） and tumor necrosis factor （TNF-α） in blood serum, at the same time expression of LBP and CD14 mRNA in liver tissues were determined with the reverse transcription polymerase chain reaction （RT-PCR）. Results： The level of plasma endotoxin and TNF-α in blood serum in the rats of model were significantly higher than normal controls （P 〈 0. 05 ）. Expression of LBP and CD14 mRNA were significantly higher than normal controls （P 〈0.05）, in liver tissue, there was marked pathological changes （steatosis, cell infiltration and necrosis）, and the level of blood serum ALT, AST, GGT, TG significantly elevate （P 〈 0. 05, plasma endotoxin and blood serum of TNF-α, GHXJYWG group were significantly lower than the model, expression of LBP and CD14 mRNA were significantly lower than the model （P 〈 0. 05） , pathology injury liver tissue notability alleviate, and the level of blood serum ALT, AST, GGT, TG signifcantly depress （P 〈 0. 05 ）. Conclusion ： GHXJYWG can prevent and cure ALD of rat injury eansed by endotoxin.