摘要
目的:探讨HGF/cMet基因表达与肝癌细胞侵袭转移能力的关系。方法:采用荧光定量PCR和WesternBlot检测5株肝癌细胞系MHCC-1、HepG2、Huh7、SMCC-7721、Hep3B和永生化肝细胞株L02以及26份肝组织中cMetmRNA和蛋白质表达水平,同时采用免疫组化法检测26份肝组织中cMet蛋白质的表达。结果:6株肝癌细胞cMetmRNA分别为7.9×106,3.4×106,2.1×106,6.5×105,3.8×105和5.8×103(copies/ml),5株肝癌细胞系cMetmRNA显著高于L02细胞系(P<0.01),其中以转移能力较强的MHCC-1细胞系的cMetmRNA表达量最高;肝癌组织、癌旁组织及周围正常组织的cMetRNA分别为2.28×107、6.80×105、5.68×105,肝癌组织的cMet基因表达明显高于癌旁及周围正常组织(P<0.01);在肝癌组织中,T1、T2、T3/T4期及NO、N1/N2的cMetmRNA分别为4.88×104、6.98×105、1.72×106、4.67×105、8.73×105,cMet的表达与肿瘤的大小及其侵袭能力明显正相关;WesternBlot和免疫组化也显示不同肝细胞系蛋白质表达水平与cMetmRNA表达一致。结论:HGF/cMet系统参与了肝癌的形成,并且在肝癌细胞的侵袭和转移的过程中起重要作用。
Objective: To investigate the correlation between the expression level of cMet and invasiveness and metastasis in hepatocelluar carcinoma. Methods: The expression level of cMet at mRNA and protein level was assessed using real time PCR on five hepatocelluar carcinoma cell lines ( MHCC-1, HepG2, Huh7, Hep3B, SMCC-7721 ) and a immortalized hepatocyte cell line (L02), 26 cases of hepatic tissues specimens including 12 hepatocelluar carcinoma tissues and 9 cases of surrounding normal tissue as well as 5 cases of normal hepatic tissues were also evaluated, Atthe same time we investigated the level of cMet protein on those 26 cases of hepatic tissues specimen by immunohistochemistry analysis. Results: The cMet mRNA copy number of 6 cell lines were 7. 9 × 10^6, 3, 4 × 10^6, 2. 1× 10^6, 6. 5 × 10^5, 3. 8 × 10^5 and 5.68 × 10^3 , respectively. Compared with L02 cell line, the expression level of cMet was found significantly more in five hepatocelluar carcinoma cell lines, among which MHCC-1 that had a potential of highly invaseness and metastasis was the most significantly (P 〈0. 01 ) ; The level of cMet mRNA of hepatocelluar carcinoma tissues was 2. 28 × 10^7, But there was 6. 80 × 10^5 and 5.8 × 10^5 in surrounding normal tissue and normal hepatic tissues ; The level of cMet of hepatocelluar carcinoma tissues was significantly higher than that of another two kinds ( P 〈 0. 01 ) ; c-Met mRNA copies were significantly correlated with the invasion; T1 versus T2, P 〈 0. 01 ; T1 versus T3/T4, P 〈 0. 01 ; T2 versus T3/T4, P 〈 0. 01 c-Met copy number in hepatocelluar carcinoma tissues of N1/N2 staged patients was significantly higher than NO cases (P 〈 0. 01 ). There was a high correlation between the level of cMet and the size of tumor as well as lymph node metastasis. Expression levels of c-Met mRNA were also verified with immunohistochemistry analysis of c-Met protein expression in hepatocelluar carcinoma tissues and surrounding normal tissue as well as normal hepatic tissues. Conclusion: HGF/cMet participate in the cancerigenesis and may play an important role in the invasiness and mesatasis of hepatocelluar carcinoma.
出处
《中西医结合肝病杂志》
CAS
2007年第2期88-91,F0003,共5页
Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases
基金
湖北省科技攻关项目(No.2005AA301C09)
关键词
癌基因
HGF/cMet
肝癌
转移
侵袭
oncogene
HGF/cMet
bepatocelluar carinoma
metastasis
invasiveness