固体发酵工艺生产杆菌肽的研究

Study on the processing conditions of bacitracin by solid-state fermentation technology

本文研究了不同固料比例及各种因素对固体发酵生产杆菌肽的影响,利用正交实验加以优化,得出其最佳生产工艺条件:豆饼粉∶玉米粉=4∶6,固料∶水=1∶1.4,葡萄糖0.8%,碳酸钙0.4%,磷酸氢二钾0.01%,泛酸钙0.01%,pH 6.0,37℃发酵36h。

Different proportion of the raw materials and important factors affecting the yield of bacitracin were studied. These important factors were optimized by the orthogonal test. The optimum fermentation processing conditions obtained as follows ：soybean power：corn power 4：6, raw materials：water 1：1.4, glucose 0.8 %, CaCO3 0.4 %, K2HPO4 0.01%, calcium pantothenate 0.01%,pH 6.0,fermentation time 36 h in 37 ℃.