用煮沸撕取法观察表皮气孔的方法

A Method for Observing Epidermis Stoma by Boiling and Tearing Epidermis from Leaf

将三年生木枣、骏枣的组培苗叶片采摘后迅速放进煮沸的水中直到叶片由翠绿变成深绿色（1～2分钟），取出后迅速撕取叶片上不同部位的表皮，将叶片表皮染色压平后迅速进行镜检。结果表明，此方煮沸撕取法比用胶带粘取法和撕取下表皮法具有操作简单、速度快、真实性强、不受材料种类的限制、定位性能强等优点，适宜于气孔基本形态和分布状况等定性指标的观察，也适宜于气孔形态学指标的定量研究，同时解决了等面叶及其表面粘有附属物（腊质层、绒毛）的叶片表皮制片难问题。

In test with the tissue culture seedlings of Ziziphus jujuba Mill vat. Muzao and Ziziphu, jujuba Junzao, 3 year-old, the picked leaves were rapidly put into boiling water for 1 - 2 min until the leaves got into deep green, then they were took out to tear up the epidermis in different position. Through dyeing and plunishing the tore epidermis were observed under microscope. Compared with the two methods of transparent gummed tape or directly tearing, the method is not only simple, rapid and accurate, but also not restricted by types of samples. The method could be used for observing conformation, distribution and morphology of stoma, as well as for quantify research of morphologic index of stoma. The method provides a simple procedure for leaf epidermis slicing for isobilateral leaves or those covered with trichomes and epicuticuular wax.