叶绿体的蛋白质组学研究进展

Progress in Chloroplast Proteomics Research

蛋白质组学是以基因组编码的所有蛋白为研究对象,高通量地从细胞及整体水平上研究蛋白质的组成及其功能的新兴学科。在后基因组时代的今天,蛋白质组学的研究正逐渐深入到生命科学的各个领域,21世纪蛋白质组学将成为生命科学中最热门的学科。蛋白质组分析已成为鉴定植物功能的有力工具之一,叶绿体作为比较重要的细胞器,在植物蛋白质组学中已有较多的研究,,随着双向电泳技术的改进和质谱法的出现,并与不断增多的拟南芥、水稻、玉米等植物的序列数据相结合,叶绿体蛋白质组可以被快速鉴定。本文主要介绍了植物蛋白质组学、叶绿体及其蛋白质组学研究技术和研究进展,并对蛋白质组学的研究趋势进行了展望。

Proteomics is a new discipline that studies proteome and a leading technology for the high-throughput analysis of proteins on a genome-wide scale. As a new research area of the post-genome era, proteomics is gradually entering every area of life science. Proteomics will be the most popular subject in the 21st century. Proteome analysis is becoming a powerful tool in the fiunctional characterization of plants. In the field of plant proteomics, chloroplast as an important organelle has been widely studied. Improvements in two-dimensional electrophoresis （2-DE） and mass spectrometry have, in combination with increasing amounts of sequence data from Arabidopsis, rice, maize and other plant species, opened up fantastic experimental possibilities enabling the chloroplast proteins to be identified rapidly. This review summarizes advances on recent proteomics studies with an emphasis on plant proteomics, chloroplast and technology of chloroplast proteomics, as well as the prospect of proteomics.