SLE患者血清中抗核小体抗体浓度与脱氧核糖核酸酶Ⅰ活性的相关分析

Analysis of Correlation Between Anti-nucleosome Antibody Concentration with DeoxyribonucleaseⅠ Activity of Serum is Systemic Lupus Erythematosus

目的:探讨系统性红斑狼疮(SLE)血清抗核小体抗体(AnuA)浓度与脱氧核糖核酸酶Ⅰ(DNaseⅠ)活性的关系。方法:采用DNA-甲基绿比色法和间接ELISA法检测76例SLE患者(活动期40例,缓解期36例)与50例健康对照组DNaseⅠ的活性和血清AnuA浓度,并分析二者之间关系。结果:SLE患者血清DNaseⅠ活性水平明显低于健康对照组(P<0.01),而疾病活动期与缓解期相比未见明显差别(P>0.05);与健康对照组相比,血清AnuA浓度升高明显(P<0.01),并且疾病活动期浓度高于缓解期(P<0.01);AnuA浓度和DNaseⅠ的活性成负相关关系(r=-0.43,P<0.01)。结论:SLE患者中DNaseⅠ的活性下降可能导致血清AnuA的浓度升高。

Objective： To explore the correlation between anti-nucleosome antibody（AnuA）with deoxyribonuclease Ⅰ （DNase Ⅰ ）activity of serum in systemic lupus erythematosus（SLE）. Methods： The serum DNase Ⅰ activity and AnuA concentration of 76 patients with SLE, 40 heathy control persons were detected by DNA-methyl green substrate conlorimetry and indirect ELISA, respectively. Meanwhile, the correlation between AnuA concentration and DNase Ⅰactivity were also analysed. Results： The serum DNase Ⅰ activity was significantly lower in SLE patients than that in healthy control （P〈0.01）; no statistical difference was found within SLE patients（P〉0.05）. Compared with healthy control, patients with SLE had higher AnuA concentration of serum （P〈0.01）; moreover, there was obvious distinction within SLE patients（P〈0.01）. The AnuA concentration was negatively correlated with DNase Ⅰ activity（r=-0.43, P〈0.01）. Conclusion： The declined DNase Ⅰ activity may make the concentration of AnuA elevate in SLE.