氨溴索对环丙沙星透过铜绿假单胞菌生物膜的影响

Effect of ambroxol on permeability of ciprofloxacin across Pseudomonas aeruginosa biofilm

目的研究氨溴索对环丙沙星透过铜绿假单胞菌生物膜的影响。方法孔径为0.22μm的医用微孔滤膜建立铜绿假单胞菌生物膜的体外模型;用高效液相色谱(HPLC)方法测定环丙沙星透过生物膜的量,并测定在2和0.75mg/ml氨溴索作用后环丙沙星透过细菌生物膜的量。结果经过7d的连续培养后,铜绿假单胞菌可在微孔滤膜上形成均匀致密的生物膜。环丙沙星可缓慢透过细菌生物膜,8h左右达到平衡;2和0.75mg/ml氨溴索作用后可以明显增加环丙沙星8h内透过生物膜的量。结论以医用微孔滤膜为载体可建立铜绿假单胞菌生物被膜体外模型;氨溴索可以显著地促进环丙沙星对细菌生物膜的透过。

Objective To investigate the effect of ambroxol on the permeability of ciprofloxacin across the bacterial biofilm of Pseudomonas aeruginosa. Methods In vitro model of P. aeruginosa biofim using 0.22μm pore diameter medical micropore film was established. The amounts of ciprofloxacin across the bact- erial biofilm was measured by HPLC under different circumstances, physiological saline, 2mg/ml and 0.75 mg/ml ambroxol, and clarithromycin was used as positive control. Results After continuous seven-day cultivation, P. aeruginosa could form compact and well-distributed biofilm on the surface of micropore film. Ciprofloxacin could slowly permeate through the biofilm and equilibration was achieved in 8h. When combined with ambroxol, the permeability rate of ciprofloxacin significantly increased. Conclusions In vitro model of P. aeruginosa biofilm was successfully established using medical micropore film as a supporter, and ambroxol can significantly promote the permeability of ciprofloxacin across the bacterial biofilm of Pseudomonas aeruginosa .