摘要
目的:采用HPLC法测定辣椒浸出物中辣椒素的含量。方法:C18色谱柱,以乙腈-水(50∶50)为流动相,检测波长为229 nm。结果:本方法重现性及精密度良好,回收率为99.4%,RSD为1.22%。结论:方法简便,专属性强;可用于辣椒浸出物的质量控制。
Objective：To develop an HPLC method for the determination of capsaicin in the ethanol extract of capsicum. Method： The chromatographic conditions were as follow： Kromasil Cls （250mm×4. 6mm, 5μm） column was used, a mobile phase composed of acetonitrilewater （50： 50） , with detection wavelength at 229nm. Result： The method showed good repetition and precision. The average recovery was 99.4%, RSD 1.22%. Conclusion ： The proposed method is simple, reliabe, can be the method for the determination of capsaicin.
出处
《药学与临床研究》
2007年第2期158-160,共3页
Pharmaceutical and Clinical Research
