白细胞介素-6基因-572G／C多态性对急性冠状动脉综合征患者血浆高敏C反应蛋白的影响

Relationship between Interleuldn-6 gene -572 G/C promotor polymorphism and plasma concentration of high sensitive C-reactive protein in patients with acute coronary syndrome

目的研究白细胞介素-6（IL-6）基因-572G／C多态性对急性冠状动脉综合征患者血浆高敏C反应蛋白（hs-CRP）浓度的影响。方法采用聚合酶链反应结合限制性内切酶片段长度多态分析方法（PCR-RFLP）检测228例急性冠状动脉综合征患者的IL-6基因-572G／C多态性，用免疫比浊法测定hs-CRP浓度。结果①ST段抬高急性心肌梗死（STEMI）组hs—CRP水平明显高于非ST段抬高急性冠脉综合征（NSTEACS）组，差异有统计学意义（P〈0．05）。②IL-6基因-572G／C多态性的基因型频率：CC42．54％、GC46．92％、GG10．52％；等住基因频率：C66．01％、T33．99％。③在STEMI组中，基因型CC组hs-CRP浓度较基因型GG＋GC组高，差异有统计学意义（P〈0．05）。多因素线性回归分析结果仍显示基因型CC携带者的血浆hs-CRP水平较携带G等住基因者高（P〈0．05）。在NSTEACS组及总人群中，不同基因型组hs—CRP比较差异无统计学意义（P〉0．05）。结论IL-6基因-572G／C多态性对急性冠状动脉综合征患者血浆hs-CRP水平无影响。在STEMI患者中，基因型CC携带者的血浆hs-CRP水平较携带G等住基因者高，提示IL-6基因-572G／C多态性在较高的炎症反应状态下，会对hs-CRP的表达产生影响。

Objective To investigate the relationship between Interleukln-6 （IL-6） gene -572 G/C promotor polymorphism and the plasma concentration of high sensitive C-reactive protein in patients with acute coronary syndrome. Methods Polymerase chain reaction combined with restriction enzyme digestion was used to detect the polymorphism of Interleukin-6 gene -572 G/C in 228 patients with acute coronary syndrome. High sensitive C-reactive protein （hs-CRP） was detted by immunoephelometric assay. Results As compared with non ST elevated acute coronary syndrome （ NSTEACS ） group, ST elevated myocardial infarction （ STEMI ） group had higher level of hs- CRP （ P 〈 0.05 ）. The genotype frequencies of IL-6 gene -572 G/C promotor polymorphism were CC 42.54% ; GC 46.92% ; GG 10.52% , and alleles frequencies were C 66.01% and T 33.99%. hs-CRP of CC genotype was higher than GC ＋ GG genotype in STEMI group （ P 〈 0.05 ）. There were no significant differences of hs-CRP in various genotypes in NSTEACS group and all subjects （ P 〉 0.05 ）. Condnsion There is no relationship between Interleukin-6 （IL-6） gene -572 G/C promoter polymorphism with the concentration of hs-CRP in patients with acute coronary syndrome. But in patients with STEMI, Subjects with CC genotype has higher level of hs-CRP than those with GG ＋ GC genotype, indicating that （ IL-6 ） gene -572 G/C promoter polymorphism will influence hs-CRP expression under higher inflammatory response.