内皮抑素联合放射治疗对裸鼠Lewis肺癌血管抑制作用的实验研究

Synergistic Inhibitory Effects of Endostatin and Radiation Therapy on Angiogenesis of Lewis Lung Cancer in Nude Mice

目的探讨内皮抑素联合放射治疗对荷Lewis肺癌小鼠的体内抑瘤效应,分析对肿瘤血管生成的抑制作用。方法32只荷Lewis肺癌的小鼠随机分为对照组、放射治疗组(1Gy/次,14d)、内皮抑素治疗组(10mg/kg,14d)和内皮抑素联合放疗治疗组,观察各组小鼠照射后不同时间肿瘤体积变化,第15天用免疫组化法检测肿瘤组织微血管密度。结果内皮抑素联合放射治疗组小鼠肿瘤体积明显小于对照组、放疗组及内皮抑素治疗组,第15天肿瘤组织微血管密度明显低于放疗组及内皮抑素治疗组。结论内皮抑素联合放射治疗抑瘤效应明显优于单纯放射治疗及内皮抑素治疗组,其机理可能是两者对抗肿瘤血管生成具有协同作用。

Objective To study the synergistic inhibitory effects of endostatin and radiation therapy on tumor growth and angiogenesis of Lewis lung cancer in nude mice. Methods 32 mice bearing Lewis lung cancer were randomly divided into control group, radiation group, endostatin group, and radiation ＋ endostatin group. The tumor growth was observed and the alteration of tumor size was calculated at different time. The tumor microvessel density was determined by immunohistochemistry assay at 15d. Results Compared with the first three groups, the tumor volume of the last group was significantly smaller, and the tumor microvessel density was significantly lower. Conclusion Endostatin combined with radiation has better synergistic inhibitory effects on Lewis lung cancer, and the mechanisms might be correlated with their synergistic effects on anti-angiogenesis.