非酒精性脂肪性肝病大鼠肝X受体α和固醇调节元件结合蛋白-1c的表达及意义

Significance and effect of liver X receptor α and sterol regulatory element binding protein-1c in non-alcoholic fatty liver disease in rats

目的观察肝X受体α(LXRα)和固醇调节元件结合蛋白-1c(SREBP-1c)在大鼠非酒精性脂肪性肝病(NAFLD)中的表达变化,以探讨二者在NAFLD形成过程中的作用及可能机制。方法建立高脂饮食诱导NAFLD大鼠模型后,采用逆转录聚合酶链反应(RT-RCR)和Western blot法动态观察NAFLD大鼠肝组织中LXRα和SREBP-1c表达变化。结果与对照组相比,NAFLD组大鼠肝组织中LXRα和SREBP-1c基因和蛋白表达,从第2周开始增加,12周时升高最显著(P<0.01),与脂肪肝进展程度一致。结论LXRα和SREBP-1c的表达变化与NAFLD的形成密切相关。

Objective To explore the significance and effect of the expression changes of liver X receptor α （LXRα） and sterol regulatory element binding protein-1c （SREBP-1c） in non-alcoholic fatty liver disease （NAFLD） in rats. Methods The rat model of nonalcoholic fatty liver disease was established with fatty diet. The mRNA and protein expression of LXRα and SREBP-1c in liver tissue were detected by reverse transcription and polymerase chain reaction （RT-PCR） and Western blot. Results Compared with Group C, in Group F, the mRNA and protein expression of LXRα and SREBP-1c in 2 week were significantly increased, and their peak was reached in 12 week （P〈0.01）. There was significantly positive correlation between the expression of them and the degree of NAFLD. Conclusion The expression changes of LXRα and SREBP-1c are closely related to the development of non-alcoholic fatty liver disease.