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复方壳多糖组织工程皮肤分泌b-FGF的初步研究 被引量：1

Pilot study on secretion of b-FGF in cultured tissue-engineered skin
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摘要 目的研究组织工程皮肤培养液中碱性成纤维细胞生长因子(b-FGF)的分泌情况。方法收集复方壳多糖组织工程皮肤制备过程中复方壳多糖皮肤替代物(SE)、复方壳多糖真皮替代物(DE)、单层角质形成细胞(KC)以及复方壳多糖DE与KC在transwell内共培养的培养液样本,双抗体夹心ELISA法测定其中b-FGF分泌量。结果b-FGF分泌量依次为单层KC<复方壳多糖DE/KC共培养<复方壳多糖SE<复方壳多糖DE。结论角质形成细胞能调控成纤维细胞分泌b-FGF能力,有利于伤口愈合。 Objective To investigate the secretion of basic fibroblast growth factor（b-FGF） in cultured tissue-engineered skin. Methods b-FGF from the composite chitosan skin equivalent（SE）, composite chitosan dermal equivalent（DE）, monolayer keratinocyte（KC） ,and DE/KC co-cultured in transwell were detected by ELISA. Results b-FGF from SE was less than that from DE, but was more than that from DE/KC co-cultured,and monolayer keratinocyte secreted the least b-FGF. Conclusion Keratinocyte can control fibroblast＇s secretion of b-FGF,which benefits the wound healing process.
作者 王亚洲 伍津津 朱堂友 李元朝 张民众
机构地区 第三军医大学大坪医院野战外科研究所皮肤科重庆市创伤愈合与组织工程研究所 重庆医科大学附属儿童医院激光美容科
出处 《重庆医学》 CAS CSCD 2007年第8期719-720,723,共3页 Chongqing medicine
基金 国家"863"计划专题资助(2003AA205022) 重庆市重点攻关课题资助(7897)
关键词 碱性成纤维细胞生长因子 组织工程皮肤 成纤维细胞 角质形成细胞 basic fibroblast growth factor 2 tissue engineered skin fibroblast keratinocvte
分类号 R318.19 [医药卫生—生物医学工程]
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参考文献11

二级参考文献25

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共引文献27

同被引文献15

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引证文献1

重庆医学
2007年 第8期

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