球孢白僵菌释放后在森林系统中定殖及持续控虫作用的证据

Evidence of survival and sustainable control on pests of Beauveria bassiana after artificial release in Massion pine plantation

在皖南马尾松林中使用球孢白僵菌无纺布菌条接种式放菌防治松褐天牛。在放菌前进行球孢白僵菌本底调查,在放菌后半年内采集该菌感染的各种僵虫,得到87株分离物。利用28SrDNA-PCRⅠ型内含子标记技术,测定放菌后释放菌株的回收率。结果表明,在87株球孢白僵菌中有66株与释放菌株的核型相同,占75.86%。半年中平均回收率为81.7%(75%-90.9%之间),这表明释放菌株已在该生态系中定殖,对松褐天牛种群发挥持续控制作用。在放菌前后球孢白僵菌种群结构有所变化,释放菌株有取代土著菌株优势地位的趋向。

In a Massion pine plantation ,non-woven fabric bands impregnated with Beauveria bassiana were used inoculatively against the pine sawyer, Monochamus alternatus. Cadavers of different insects were collected before and after the fungal release ,and 28S rDNA group I intron markers were used to detect recovery of the released strain. Among 87 isolates collected after release ,66 were identical to the released strain, accounting for 75.86%. In 6 months after release, recovery of the released strain varied within 75%-90.9%; with an average of 81.74%. Thus, the released strain colonized in the pine ecosystem and provided sustainable sawyer control. Population structure changed after the release, and the released strain took over the dominant position originally by indigenous type isolates.