人结肠癌组织中COX-2及nm23-h1的表达及临床意义

The expressions and clinical significance of COX-2 and nm23-h1 in human colon cancer

目的研究结肠癌、结肠腺瘤和癌旁正常结肠黏膜组织中环氧化酶2(COX-2)及nm23-h1的表达及其临床意义。方法85例结肠癌、18例结肠腺瘤和9例癌旁结肠黏膜组织分别制成72点和104点两块组织芯片,采用免疫组织化学法检测结肠组织芯片中COX-2及nm23-h1的表达。结果COX-2在结肠癌、结肠腺瘤和正常结肠黏膜组织中的表达差异显著(P<0.01),结肠癌组织中C0X-2的表达与结肠癌分化程度、淋巴结和远处转移以及Dukes分期有关,与年龄和性别无关。nm23-h1的表达在结肠癌、结肠腺瘤和正常结肠黏膜组织中差异有显著性(P<0.01),结肠癌组织中nm23-h1的表达与分化程度、远处转移及Dukes分期有关,但与其它临床病理资料无关。结论COX-2异常表达可能是结肠癌发生的早期事件,且在结肠癌的发生、发展中起一定作用。nm23-h1的表达下调可促使结肠癌细胞获得进一步恶性分化及远处转移的潜能。

Objective To study the expressions and clinical significance of COX-2 and nm23-h1 in human colon cancer, adenomatous polyps and normal para -cancer colon mucosa. Methods Eighty-five cases of colon cancer, 18 cases of adenomas and 9 cases of para-cancer mucosa were accumulated and made into two tissue microarrays containing 72 dots and 104 dots, respectively. Expressions of COX-2 and nm23-h1 proteins were detected by immunohistochemical staining. Results Significant differences in the expressions of COX-2 were found among colon cancer, adenomas and benign paracancer mucosa（P〈0. 01）. There was a positive correlation of COX-2 expression with histological grading, lymph node metastases, distance metastases and Dukes stages. The statistical significances were also found among colon cancer, adenomas and benign para-cancer mucosa in the expressions of nm23-h1 （P〈0. 01）, which showed that expression ratios in cancer and adenoma were higher than those in adjacent normal mucosa. In addition, expression of nm23-h1 had no correlation with other clinical pathological parameters but histological grading （P〈0. 05）, distance metastasis （P〈0. 01） and Dukes stage （P〈0. 05）. Conclusion Abnormal expression of COX-2 may be the early events in colon carcinogenesis and play pivotal roles in the progression of colon cancer. The colon cancer cells with down-regulated expression of nm23-h1 will acquire a possibility of further differentiation and distant metastasis.