老年人食管鳞状细胞癌、腺癌和化生-不典型增生-腺癌微卫星改变

Microsatellite Alterations in Elderly Patients with Esophageal Squamous Cell Carcinoma, Adenocarcinoma or Metaplasia-Dysplasia-Adenocarcinoma Sequence

目的评估老年人食管鳞状细胞癌(ESCC)、食管腺癌(EADC)和Barrett化生-不典型增生-腺癌序列DNA微卫星的改变。方法应用稀释性PCR技术检测老年人ESCC、EADC和Barrett化生-不典型增生-腺癌序列中D2S123、D3S1616、D3S1300、BATRII、D5S346、D17S787和D18S617个位点DNA微卫星的改变。结果在非稀释DNA中,23例老年人ESCC和18例老年人EADC微卫星不稳定性(MSI)的频率分别是47.8%(11/23例)和38.9%(7/18例),杂合性丢失(LOH)的频率分别是26.1%(6/23例)和16.7%(3/18例),两者的MSI或LOH频率比较没有显著差别(P>0.05)。在稀释DNA中,8例老年人正常食管鳞状上皮和Barrett化生-异型增生-腺癌序列的MSI和LOH频繁出现,尤其在D3S1616、D2S123、D3S1300和D17S787位点上,与非稀释DNA的MSI和LOH频率相比有显著差别(P<0.05)。结论老年人ESCC和EADC微卫星改变没有差别。老年人正常食管鳞状上皮和Barrett化生-异型增生-腺癌组织DNA的MSI和LOH普遍存在,它们可能是EADC发生发展的早期分子事件,D3S1616、D2S123、D3S1300和D17S787位点的微卫星改变可能在此过程中起着重要作用。

Objective To assess microsatellite alterations in elderly patients with esophageal squamous cell carcinoma（ESCC）, esophageal adenocarcinoma（EADC） and metaplasia dysplasia adenocarcinoma sequence. Methods Microsatellite analysis were evaluated at D2S123, D3S1616, D3S1300, BATRII, D5S346, D17S787, and D18S61 loci. The occurence of microsatellite instability（MSI） and rates of loss of heterozygosity（LOH） was detected in elderly patients with ESCC, EADC and metaplasia dysplasia adenocarcinoma sequence. Results The rates of MSI are 47.8%（11/23 cases） and 38.9%（7/18 cases） at undilution DNA in 23 cases ESCC and 18 cases EADC. The rates of LOH are 26. 1%0（6/23 cases） and 16.7%（3/18 cases） at undilution DNA in ESCC and EADC. There are not statistically significant differences in the rates of MSI and LOH at above 7 loci at undilution DNA between ESCC and EADC（P〉0.05）. The rates of MSI and LOH at above 7 loci are frequent at dilution DNA in 8 cases normal esophageal squamous epithelia and metaplasia dysplasia adenocarcinoma sequences, especially at D3S1616, D2S123, D3S1300 and D17S787 loci. There are statistically significant differences in the rates of MSI and LOH between dilution DNA and undilution DNA. Conclusion There are not differences in the rates of MSI and LOH between ESCC and EADC in elderly patients. High level rates of MSI and LOH are common at dilution DNA in elderly patients with normal esophageal squamous epithelium and metaplasia dysplasia adenocarcinoma sequence. The MSI and LOH may be a early genetic events during esophageal carcinogenesis.