抑癌基因fhit及凋亡抑制蛋白survivin在宫颈鳞状细胞癌的表达及意义

Expression and Clinical Significance of Fhit and Survivin Protein in the Tissue of Cervical Squamous Carcinoma

目的 探讨抑癌基因fhit和凋亡抑制蛋白survivin在宫颈鳞状细胞癌（鳞癌）及宫颈上皮内瘤变（CIN）的表达与意义及其相关性.方法 采用免疫组织化学技术（SP法）检测82例宫颈鳞癌和40例CIN以及20例慢性宫颈炎中fhit蛋白和survivin蛋白的表达,并结合临床病理特征进行分析.结果 （1）fhit基因在宫颈鳞癌组织中的阳性表达率为32%,与慢性宫颈炎及CIN的差别有统计学意义（P＜0.01）,与宫颈鳞癌的病理分级（G1～3）、临床分期（Ⅰ～Ⅲ）、患者年龄和有无淋巴转移无关（P＞0.05）.（2）survivin蛋白在宫颈鳞癌组织中的阳性表达率为69.51%,与慢性宫颈炎及CIN的差别有统计学意义（P＜0.01）,并随宫颈鳞癌的临床分期（Ⅰ～Ⅲ）递增（P＜0.05）,而且与患者年龄、有无淋巴转移有关（P＜0.05）,与病理分级无关（P＞0.05）.（3）fhit基因的失活和survivin蛋白的诱导在宫颈癌组织中的表达呈低度相关（P＜0.05）.结论 fhit基因的失活和survivin蛋白的诱导在宫颈鳞癌的发生发展中起重要作用,可能为宫颈鳞癌的诊疗提供新的靶点.

Objective To study the aberrant expression and coexpression of protein fhit and survivin in cervical squamous carcinoma and cervical intraepithelial neoplasia. To examine the relationship between the fhit expression and the survivin expression in cervical squamous carcinoma. Methods Immunohistochemical staining of fhit and survivin were performed by the standard streptavidin-peroxidate （SP） technique for the paraffin-embeded sections of 82 cervical squamous carcinomas and 40 cervical intraepithelial neoplasia（CIN）. The relationship between expression of fhit and survivin with the clinicopathologic parameters, prognosis was analyzed, respectively. Results （1）The positive rate of cervical carcinoma, CIN and chronic cervicitis were 32% （26/82）, 80%（32/40） and 85%（17/20） respectively; the positive rate of fhit was lower than those in CIN and chronic cervicitis（P〈0.01）. （2）The positive rate of survivin protein were 69.51% （57/82）, 30 % （12/40） and 15 % （3/20） in cervical squamous carcinoma, CIN and chronic cervicitis（P〈0.01）. Conclusion Depression of fhit gene up-regulation survivin expression may play synergetic roles in pathogenesis of cervical squamons carcinoma. It may be helpful to making a diagnosis of or screening for cervical carcinoma.