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12-脂氧化酶影响胃癌细胞株AGS蛋白激酶C活性 被引量：2

Effect of 12-Lipoxygenase Activation of PKC in Human Gastric Cancer Cell Line AGS
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摘要 目的研究胃癌细胞中蛋白激酶C(PKC)活化与12-脂氧化酶(12-LOX)的关系。方法胃癌细胞株AGS于含10%胎牛血清的RPMI1640培养液中常规培养,AGS细胞培养24h至对数生长期改用无血清RPMI1640培养基培养24h加入干预药物。PKC活性采用非同位素标记法测定。分别采用12-LOX催化合成产物12-羟基廿碳四烯酸(12-HETE)及12-LOX特异性抑制剂——黄芩素干预AGS细胞,测定其PKC活性变化。结果AGS细胞经12-HETE干预后PKC活性明显增高,而经黄芩素干预后PKC活性明显下降,且呈时间、剂量依赖性。结论12-LOX对PKC活性具有调节作用;PKC参与了12-LOX影响胃癌细胞增殖凋亡的信号传导。 Objective To investigate the effects of 12-lipoxygenase （12-LOX） and its inhibitor, baicalein, on PKC activation in human gastric cancer cell AGS. Methods Human gastric cancer cell AGS were cultured in a RPMI 1640 medium with 10% fetal bovine serum（FBS）. After overnight incubation, AGS cells were cultured in a fresh RPMI 1640 medium without FBS for another 24 h and then treated with 12-LOX inhibitor, baicalein, or its metabolite 12-HETE respectively in various concentrations. The activity of PKC was examined by PePTag non-radioactive protein kinase assay. Results The PKC activity was increased after 12-HETE cocultured（P〈0.05） and decreased after baicalein（P〈0.05） in a time and concentration dependent manner. Conclusion 12-LOX affects the activity of PKC in gastric cancer cell line AGS. PKC is involved in the proliferation or apoptosis procedure mediated by 12-LOX in AGS cells. 12-LOX inhibitor may contributed an anticancer affect in human gastric carcinoma.
作者 陈丰霖 王小众 李建英 黄彩云 陈治新
机构地区 福建医科大学附属协和医院消化科
出处 《福建医科大学学报》 2007年第2期131-133,共3页 Journal of Fujian Medical University
基金 福建省教育厅科研基金资助项目(JA03094)
关键词 胃肿瘤 脂氧合酶 蛋白激酶C 肿瘤细胞 培养的 stomach neoplasms lipoxygenase protein kinase C tumor cells,cultured
分类号 R735.2 [医药卫生—肿瘤]
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参考文献14

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共引文献46

同被引文献25

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福建医科大学学报
2007年 第2期

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