沙土鼠短时氧惊厥海马各区神经元Bcl-2表达的变化

Changes in the expression of neuron Bcl-2 in hippocampns following transient hyperbaric oxygeninduced convulsion in gerbils

目的观察短时氧惊厥沙土鼠脑海马各区神经元Bcl-2表达的变化，为氧惊厥的防治提供理论依据。方法应用免疫组化LSAB染色法，研究沙土鼠暴露在0．5MPa压力的高压氧下，引起短时氧惊厥（首次惊厥5min后，减压出舱）后脑海马各区神经元Bcl-2表达情况。结果沙土鼠在发生短时氧惊厥后3d，氧惊厥组海马各区神经元Bcl-2蛋白表达阳性，CA3区表达最强，其次为CA4、CA4、CA1区，而正常对照组及压力对照组动物海马区未见阳性细胞。结论短时氧惊厥诱导海马各区神经元Bcl-2表达，可能是海马神经元抗氧惊厥损伤的细胞内保护性机制在起作用。

Objective To observe the effect of transient hyperbaric oxygen-induced convulsion （HBOC） on the expression of Bcl-2 in different regions of gerbil hippocampus, and to provide theoretic basis for the treatment of HBOC. Methods The expression of Bcl-2 in different regions of gerbil hippocampus following transient HBOC, in which gerbils were exposed to 0.5 MPa hyperbaric oxygen for 5 min after first generalized convulsion, were examined by LSAB immunohistochemical staining method. Results The expression of protein Bcl-2 was found in different regions of gerbil hippocampus 3 d after HBOC. It was the highest in CA3 region, and next in CA4, CA2, and CA1 regions （ P 〈 0. 01 ） in order. Conclusions Bcl-2 induced by HBO is probably effective in protecting neurons from injury.