晚孕期胎盘因子对小鼠淋巴细胞增殖的影响

Influences of the 3rd trimester placental factor on mouse lymphocytes proliferation

通过晚孕期胎盘因子(PF)对小鼠淋巴细胞体外增殖的作用,探讨PF对正常机体免疫系统和免疫功能的效应。分离小鼠淋巴细胞,用ConA刺激其增殖,并分别与晚孕期不同浓度的PF作用,于48 h和72 h用活体染料羧基荧光素乙酰乙琥珀酰亚胺酯(CFSE)标记,流式细胞术检测增殖。于48 h碘化丙啶染色,流式细胞术检测细胞周期分布。结果显示,晚孕期PF在48 h和72 h时间点均能显著促进ConA诱导的小鼠淋巴细胞增殖反应,且效应与剂量呈正相关。晚孕期各浓度PF组G0期所占比率均显著低于对照组,且浓度越大,比率越低,组间两两比较有统计学意义。各浓度PF组S期所占比率均显著高于对照组,各浓度PF组G2/M期所占比率均显著高于对照组,25%PF组G2/M期所占比率均显著高于5%PF组和15%PF组。晚孕期PF通过促进小鼠淋巴细胞由G1期向S和G2/M期而促进ConA刺激小鼠淋巴细胞的增殖反应,提示PF具有免疫调节的功能,可以提高机体的免疫功能。

To study the effects of the 3rd trimester placental factor （PF） on mouse lymphocytes proliferation, and to explore their possible role in immune function. ConA stimulated mouse lymphocytes were treated with different concentrations of the 3rd trimester PF, and then labeled with CFSE or propidium iodide. Lymphocytes proliferation was measured at 48 h and 72 h and cell cycle distribution was examined at 48 h with flow cytometry. The PF at diffecent concentrations increased significantly the ConA-stimulated mouse lymphocytes proliferation in a dose-dependent manner. The percentages of GO of all 3rd trimester PF groups were significantly lower than that of the control group respectively, while the percentages of S were significantly higher when compared with the control group. The percentages of G2/M of all 3rd trimester PF groups were significantly higher than that of the control group, and the percentage of G2/M of 25% PF group was significantly higher than those of 5% and 15% PF groups respectively. It is evident that the 3rd trimester PF can enhance mouse lymphocytes proliferation, and thus improving the immune function.