两种交联处理后猪小肠黏膜下层单轴拉伸性能比较

Uniaxial tensile mechanical properties of porcine SIS following different cross-linking treatments

目的寻找一种理想的猪小肠黏膜下层(small-intestinal submucosa,SIS)交联处理方法。方法将来自同一个体的猪SIS分别进行戊二醛处理和亚甲基蓝光氧化交联处理,与新鲜组用相同的方法在万能实验机上做沿管腔纵向单轴拉伸测试,并对极限抗张强度σ_(max)、断裂应变ε_m和0．3MPa应力下的弹性模量(E)处理研究。结果三组极限抗张强度分别为(6．01±1．43)、(6．96±0．93)和(12．94±2．03)MPa,戊二醛处理后的SIS在强度上有较大改善,但是组织变得僵硬;亚甲基蓝光氧化交联处理后的SIS强度改善不是特别明显,柔韧性增强。结论该研究首次使用戊二醛交联和亚甲基蓝光氧化交联方法对猪SIS进行了处理,并比较了与新鲜猪SIS沿管腔纵向单轴拉伸测试力学性能的差异,并证明可以发展光氧化交联方法使其成为一种有效的SIS交联手段。

Objective As a new graft biomatedal for tissue engineedng, small-intestinal submucosa （SIS） has an excellent biomechanical property. This study aims to investigate an ideal method for crosslinking porcine small-intestinal submucosa. Methods Stabilizing porcine SIS by Glutaraldehyde（GA） and photofixing samples from the same animal with methylene blue, then testing them together with original samples by matedal test machine. Data were obtained and analyzed, including ultimate tensile stress （σmax）, breaking strain （εm）, and modulus of elasticity（E） under 0.3MPa stress. Results The ultimate tensile stress of three groups are respectively 6.01±1.43. 6.96±0.93 and 12.94±2.03MPa. Samples following GA treatment improve correspondingly, but stiffness of the tissue is increased. Intensity of samples following photofixing with methylene blue improve slightly, and flexility improve. Conclusion This study stabilized porcine SIS by GA and methylene blue for the first time, and through comparing their biomechanical properties of uniaxial tensility, it was testified that the method of photooxidation shall be developed as a valid treatment to crosslink SIS.