基质金属蛋白酶MMP-2在龋病发展过程中的表达研究

Expression of MMP-2 in pulp of healthy and carious tooth

目的:观察基质金属蛋白酶MMP-2在健康及不同程度龋损牙齿牙髓中的表达,初步研究MMP-2与龋病发展的关系。方法:以健康、浅龋和深龋牙齿中牙髓组织为研究对象,利用免疫组化、RT-PCR、实时荧光定量PCR和Western印迹检测MMP-2在其中的表达差异。采用单因素方差进行统计学分析。结果:组织标本免疫组化显示MMP-2在健康组和浅龋组中呈弱阳性表达,在深龋组中则表达强阳性。在健康组中仅表达于成牙本质细胞中,而在龋病组中牙髓细胞亦有表达。实时荧光定量PCR和Western印迹均检测到MMP-2在深龋组中表达明显高于其他两组,健康组和浅龋组表达无明显差异。结论:MMP-2的表达部位和强度与龋病的进展相关。

AIM：To investigate the expression of matrix metalloproteinase- 2 （MMP- 2 ） in the pulp of the healthy and carious tooth and to evaluate the relationship between the expression of MMP - 2 and caries developement. METHODS ： The expression of MMP - 2 in of the pulp of the healthy and carious tooth were examined by immunohistochemical staining, RT - PCR, real - time PCR and western - blot. One - way ANOVA was used for statistical analysis. RESULTS： By immunohistochemical staining, MMP -2 expressed strongly in deep caries while weakly in the other two groups and expressed in both odontablast and pulp cells of the tooth with caries, but only in odontalblast when the tooth was healthy. RT - PCR, real - time PCR and western - blot confirmed that MMP - 2 expressed in deep caries group was significantly higher than in the other two groups（ P 〈 0.01 ）. CONCLUSION： The expression of MMP - 2 may contribute to caries development.