二仙汤对去卵巢大鼠骨质疏松的作用

Protective effect of Er-xian decoction on ovariectomy-induced osteoporosis in rats

目的:研究二仙汤对去卵巢大鼠骨质疏松症的作用。方法:去卵巢诱导大鼠骨质疏松症,12周后,取胫骨近端行不脱钙制片进行骨组织形态计量学分析;双能X射线骨密度仪测量股骨骨密度。结果:二仙汤能显著增加骨小梁面积百分比和骨小梁厚度(P<0.01),减少骨小梁分离度(P<0.05);增加骨形成参数:矿化沉积率(MAR)、骨形成率(BFR/BS、BFR/BV、BFR/TV)(P<0.05,P<0.01);减少破骨细胞数(P<0.01)且显著增加去卵巢大鼠股骨骨密度(P<0.05)。结论:二仙汤对去卵巢大鼠骨质疏松有保护作用,主要是通过促进骨形成、抑制骨吸收、提高骨密度。

Objective：To investigate the protective effects of Er-xian decoction on ovariectomy-induced osteoporosis in rats. Methods.. The osteoporosis model was induced by ovariectomy in rats. Twelve weeks later, the undecalcified longitudinal proximal tibial metaphysical sections were made and stained for the bone histomorphometric analysis. Bone density of femur metaphysis was determined by dual-energy X-ray absorptiometry. Results： Er-xian decoction significantly increased trabecular area and trabecular thickness （P〈0.01） and decreased trabecular separation（P〈0.05）. The parameters of bone formation of rats, such as MAR, BFR/BS, BFR/BV, and BFR/TV, were increased significantly after Er-xian decoction treatment（P〈 0. 05, P〈0.01）. Moreover, Er-xian decoction obviously reduced osteoclast number（P〈0.01）and enhanced bone density of femur metaphysis in ovariectomized rats （P 〈 0. 05 ）. Conclusion： Er-xian decoction has antiosteoporotic effects on ovariectomized rats by promoting bone formation, increasing bone density, and inhibiting bone resorption.