期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

重组C反应蛋白促进脐静脉内皮细胞表达基质金属蛋白酶2 被引量：2

C-Reactive Protein Enhances the Expression of Matrix Metalloproteinase-2 in Human Umbilical Vein Endothelial Cells
下载PDF
导出
摘要 目的观察重组C反应蛋白(CRP)对人脐静脉内皮细胞(HUVEC)基质金属蛋白酶2(MMP-2)表达的影响。方法采用逆转录聚合酶链反应及蛋白质免疫印迹分析检测MMP-2mRNA转录和蛋白水平表达,观察不同浓度CRP及不同孵育时间对HUVEC MMP-2表达的影响。结果CRP呈浓度和时间依赖性增加培养HUVEC的MMP-2mRNA转录和蛋白水平的表达。结论CRP可上调HUVEC MMP-2表达。 Objective To investigate the effects of C-reactive protein（CRP） on expression of matrix metalloproteinase-2 （ MMP-2） in human umbilical vein endothelial cells （ HUVEC）. Methods HUVEC were cocultured with different concentration of CRP for different time. MMP-2 protein and mRNA expression in HUVEC were measured by Western blotting and reverse transcription polymerase chain reaction（RT-PCR）. Results Incubation of HUVEC with CRP results in an increasing expression of protein and mRNA of MMP-2 in a concentration and time-depedent manner. Conclusion CRP enhances the expression of MMP-2 in HUVEC.
作者 陈春燕 高玲 陈琴 康丽娜 徐标
机构地区 南京大学医学院附属鼓楼医院心内科
出处 《中华高血压杂志》 CAS CSCD 北大核心 2007年第3期199-202,共4页 Chinese Journal of Hypertension
基金 江苏省自然科学基金(BK2004083) 国家自然科学基金(NO 30170370)资助
关键词 C反应蛋白 脐静脉内皮细胞 基质金属蛋白酶 动脉粥样硬化 C-reactive protein Human umbilical vein endothelial cells Matrix metalloproteinase
分类号 R543 [医药卫生—心血管疾病]
  • 相关文献

参考文献16

  • 1[1]Danesh J,Wheeler JG,Hirschfield GM,et al.C-reactive protein and other circulating markers of inflammation in the prediction of coronary heart disease[J].N Engl J Med,2004,350:1387-1397.
  • 2[2]Wilson AM,Ryan MC,Boyle AJ.The novel role of C-reactive protein in cardiovascular disease:risk marker or pathogen[J].Int J Cardiol,2006,106:291-297.
  • 3[3]Meuwissen M,van der Wal AC,Niessen HW,et al.Colocalisation of intraplaque C reactive protein,complement,oxidized low density lipoprotein,and macrophages in stable and unstable angina and acute myocardial infarction[J].J Clin Pathol,2006,59:196-201.
  • 4[4]Li Z,Li L,Zielke HR,et al.Increased expression of 72 kD type Ⅳ collagenase (MMP-2) in human aortic atherosclerotic lesions[J].Am J Pathol,1996,148:121-128.
  • 5丁巍,魏渝娟,万德中,左云慧,张浩,禄秀秀.急性冠状动脉综合征患者血清基质金属蛋白酶-2与C-反应蛋白水平[J].临床心血管病杂志,2006,22(4):217-219. 被引量：14
  • 6[6]Pasterkamp G,Schoneveld AH,Hijnen DJ,et al.Atherosclerotic arterial remodeling and the localization of macrophages and matrix metalloproteinases 1,2 and 9 in the human coronary artery[J].Atherosclerosis,2000,150:245-253.
  • 7[7]Kai H,Ikeda H,Yasukawa H,et al.Peripheral blood levels of matrix metalloproteases-2 and-9 are elevated in patients with acute coronary syndromes[J].J Am Coll Cardiol,1998,32:368-372.
  • 8[8]Pasceri V,Willerson JT,Yeh ET.Direct proinflammatory effect of C-reactive protein on human endothelial cells[J].Circulation,2000,102:2165-2168.
  • 9[9]Pasceri V,Chang J,Willerson JT,et al.Modulation of C-reactive protein-mediated monocyte chemoattractant protein-1 induction in human endothelial cells by anti-atherosclerosis drugs[J].Circulation,2001,103:2531-2534.
  • 10[10]Verma S,Li SH,Badiuala MV,et al.Endothelin antagonism and interleukin-6 inhibition attenuate the proatherogenic effects of C-reactive protein[J].Circulation,2002,105:1890-1896.

二级参考文献12

  • 1葛智平,陈晓春.C反应蛋白与不同类型冠心病相关性的临床研究[J].临床心血管病杂志,2005,21(2):78-80. 被引量：37
  • 2TORZEWSI J, TORZEWSKI M, BOWYER D F, et al. C-reactive Protein frequently colocalizes with the terminal complement complex in the intima of early atherosclerotic lesions of human coronary arteries[J].Arterioacler Thromb Vasc Biol, 1998, 18: 1386-1392.
  • 3HOFFMEISTER H M, EHLERS R, BUTTTTCHER E,et al. Comparison of C-reactive Protein and terminal complement complex in Patients with unstable angina Pectoris versus stable angina pectoris [J]. Am J Cardiol,2002,89:909- 912.
  • 4RIDKER P M ,CUSHMAN M ,STEMPFER M J, et al. Infammation aspirin and the risk of cardoivascular diseases in apparently healthy men [J]. N Engl J Med, 1997,336 :973-979.
  • 5MORISHIGE K, SHIMOKAWA H, MATSUMOTO Y, et al. Overexpression of matrixmetalloproteinase-9 promotes intravaseular thrombus formation in porcine coronary arteries in vivo [J]. Cardiovase Res, 2003,57:572-85.
  • 6BROWN D L, HIBBS M S, KEARNEY M, et al. Identifieation of 92-kD gelatinase in human coronary atheroselerotie lesions. Association of active enzyme synthesis with unstable angina [J]. Circulation,1995,91 :2125-2131.
  • 7SHAH P K, FALK E, BADIMON J J, et al. Human monocyte-derived macrophages induce collagen breakdown in fibrous caps of atherosclerotic plaques. Potential role of matrix-degrading metalloproteinases and implications for plaque rupture [J]. Circulation,1995,92:1565- 1569.
  • 8KAI H, IKEDA H, YASUKAWA H, et al. Peripheral blood levels of matrix metalloproteases-2 and-9 are elevated in parients with acute coronary syndromes[J]. J Am Coll Cardiol, 1998,32 : 368- 372.
  • 9HOJO Y, IKEDA U, UENO S, et al. Expression of matrix metalloproteinases in patients with acute myocardial infarction [J]. Jpn Circ J, 2001,65:71 - 75.
  • 10BLANKENBERG S, RUPPRECHT H J, POIRIER O, et al. Plasma concentrations and genetic variation of matrix metalloproteinase 9 and prognosis of patients with cardiovascular disease[J]. Circulation,2003,107: 1579- 1585.

共引文献13

同被引文献28

  • 1张承俊,朱兴雷.辛伐他汀对肿瘤坏死因子α诱导的人脐静脉内皮细胞基质金属蛋白酶9表达的影响[J].中国动脉硬化杂志,2006,14(1):50-52. 被引量：5
  • 2Senzaki H. The pathophysiology of coronary artery aneurysms in kawasaki disease: role of matrix metalloproteinases[J]. Arch Dis Child, 2006,91 (10) : 847 - 851.
  • 3Senzaki H, Masutani S, Kobayashi J, et al. Circulating matrix metalloproteinases and their inhibitors in patients with Kawasaki disease[J]. Circulation, 2001, 104(8): 860- 863.
  • 4Lau AC, Rosenberg H, Duong TT, et al. Elastolytic matrix metalloproteinases and coronary outcome in children with kawasaki disease[J]. Pediatr Res, 2007,61(6):710 -715.
  • 5Gavin PJ, Crawford SE, Shulman ST, et al. Systemic atterial expression of matrix metalloproteinases 2 and 9 in acute Kawasakidisease [ J ]. Arterioscler Thromb Vasc Biol, 2003,23(4) :576 - 581.
  • 6Lau AC, Duong TT, Ito S, et al. Matrix metallopmteinase 9 activity leads to elastin breakdown in an animal model of Kawasaki disease[J]. Arthritis Rheum, 2008,58(3):854 - 863.
  • 7Wang BW, Chang H, Lin S. Induction of matrix metalloproteinases-14 and -2 by cyclical mechanical stretch is mediated by tumor necrosis factor-alpha in cultured human tanbilical vein endothelial cells [ J]. Cardiovasc Res, 2003,59(2) :460 - 469.
  • 8Hui Yuen JS, Duong TF, Yeung RS, et al. TNF-alpha is necessary for induction of coronary artery inflammation and aneurysm formation in an animal model of Kawasaki disease[J].J Immunol, 2006,76(10) :6294 - 6301.
  • 9Kaneko K, Savage CO, Pottinger BE, et al. Antiendothelial cell antibodies can be cytotoxic to endothelial cells without cytokine pre-stimulation and correlate with ELISA antibody measurement in kawasaki disease [ J]. Clin Exp Immunol, 1994,98(2) :264 - 269.
  • 10Sakata K, Kita M, lmanishi J, et al. Effect of Kawasaki disease on migration of human umbilical vein endothelial cells[J]. Pediatr Res, 1995,38(4) :501 - 505.

引证文献2

二级引证文献14

中华高血压杂志
2007年 第3期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部