血管瘤组织中TRAIL蛋白及其mRNA的表达和意义

Expression and significance of TRAIL protein and mRNA in hemangioma

目的 检测血管瘤组织中TRAIL（肿瘤坏死因子相关凋亡诱导配体）蛋白及其mRNA的表达，并探讨其意义。方法 采用免疫组织化学和原位杂交方法检测血管瘤增殖期、退化期、血管畸形和正常皮肤组织中TRAIL蛋白和mRNA的表达。结果 TRAIL蛋白和mRNA在血管瘤增殖期和退化期中的阳性表达率分别为45．45％（15／33）和78．57％（22／28），66．67％（22／33）和89．29％（25／28），血管畸形和正常皮肤组织中均为阴性。秩和检验分别为Hc=54．03，Hc=71．82，四者之间差异均有统计学意义（P〈0．01），两两之间比较，增殖期与退化期之间差异均有统计学意义（P〈0．01）。结论 TRAIL可能通过诱导内皮细胞凋亡，促进血管瘤的消退。

Objective To detect the expression of TRAIL protein and mRNA in hemangiomas and vascular malformations. Methods Sections of 33 proliferative hemangiomas,28 involuting bemangiomas and 29 vascular malformations were immunostained for TRAIL protein, TRAIL mRNA was examined by in situ hybridization in these tissue. Results The TRAIL protein positive rates in proliferative bemangiomas, involuting hemangiomas, vascular malformations and normal skins were respectively 45.45 % （ 15/33 ）, 78.57 % （ 22/28 ）, 0 % and0 % . There were significant differences among the four pathologies （ P 〈 0.01 ）, The difference between proliferative hemangiomas and involuting hemangiomas was also significant （ P 〈 0.01 ）. The TRAIL mRNA positive rates were 66.67% （ 11/33 ）, 89.29 （25/28）, 0% and 0% respectively. There were also significant differences among the four pathologies （P 〈 0.01 ）, The difference between proliferative hemangiomas and involuting hemangiomas was also significant （P 〈 0.01 ）. Conclusions TRAIL could induce endothelial apoptosis and cause regression of hemangiomas.