类固醇类激素联合携带Fas基因重组腺病毒治疗瘢痕疙瘩的体内实验研究

Experimental gene therapy of keloid in vivo using recombinant adenovirus coding for Fus gene with steroid hormone

目的 应用成功制备的携带人Fas基因的两种重组腺病毒，联合类固醇激素进行瘢痕疙瘩的动物实验研究，判断携带人Fas基因的重组腺病毒与类固醇激素联合治疗瘢痕疙瘩的效果。方法 构建瘢痕疙瘩裸鼠模型。使用Ad-Fas（B），Ad-Fas（T）两种构建成功的腺病毒注射及其它辅助治疗手段，实施针对植入裸鼠皮下的瘢痕疙瘩组织的体内治疗方案。通过大体观察，常规病理及电镜观察检测瘢痕疙瘩组织块的变化。结果 ①单纯使用腺病毒注射后的瘢痕疙瘩组织块体积仅轻度缩小。②前期注射Ad-Fas（B）或Ad-Fas（T）后，使用类固醇激素作为后续治疗因素，其瘢痕疙瘩组织块均明显缩小。③使用腺病毒治疗后，能有效地减少曲安缩松的使用，从而减轻类固醇类激素治疗的副作用。结论 ①裸鼠为免疫缺陷动物，所以该结果并不能否认病毒的直接治疗作用，在免疫性动物体内直接注射腺病毒的治疗效果尚无结论。②重组腺病毒Ad-Fas（B）及Ad-Fas（T）的瘢痕疙瘩基因治疗的动物实验为瘢痕疙瘩的治疗展示了一条全新的途径。

Objective To observe the gene therapeutic effects in vivo using prepared recombinant adenovirus coding for Fas gene with steroid hormone. Methods Transplant the keloid tissue into athymic nude mice and then observe the tissue＇ s change after injecting Ad-fas（T） or Ad-Fas（B） followed by steroid hormone. Results ① The keloid tissue shrinked slightly when adenovirus was singly injected. ②The keloid tissue transplanted into athymic nude mice was seen significantly shrink after injection of Ad-Fas（T） or Ad-Fas（B） followed by steriod hormone, ③The injection of Ad-Fas（T） or Ad-Fas（B） may be helpful to decrease the dose of steroid hormone which may significant depressed its side-effect. Conclusions ODue to the immune deficiency in nude mice, the conclusion can not be made that the single injection of adenovirus is invalid. ②Gene therapy in vivowas also successfully completed using the newly built Ad-Fas（T） and Ad-Fas （B）. The results were satisfactory. The success in experimental study may pave a sound foundation for further gene therapy in keloid.