甲氧滴滴涕对孕鼠生殖及其胎鼠发育的影响

Reproductive and developmental toxi-city of methoxychlor in pregnant mice and their foetuses

目的:研究甲氧滴滴涕(MXC)染毒对孕小鼠生殖和胎鼠发育的影响.方法:将交配成功的雌鼠于孕12～17d每日给予不同剂量的MXC(0,20,100,200mg/kg)灌胃,并设芝麻油溶剂为对照组,观察各组孕鼠体质量和黄体等计数测定,同时观察各组F1子代仔鼠的体质量和肛门生殖器距离等,采用放射免疫法检测孕鼠血清雌二醇(E2)和孕酮(P)含量.结果:生殖影响:随染毒剂量增加,母鼠子宫着床数减少,雄性胎鼠肛殖距(AGD)缩短,孕鼠血清中E2和P水平升高,呈不同剂量间差异,具有统计学意义(P<0.05).发育影响:随染毒剂量增加孕鼠体质量增长减少,活胎数下降,不良妊娠结局(死胎、吸收胎数)增多,呈不同剂量间差异,具有统计学意义(P<0.05).结论:MXC可干扰孕小鼠的生殖功能,导致生殖和胎仔发育毒性.

AIM： To explore the effects of methoxychlor （MXC） on reproduction and development in mice. METHODS： The pregnant mice were administered by oral gavage with MXC at different doses of 0, 20, 100 and 200 mg/kg during day 12 -17 of pregnancy. Meanwhile, other pregnant mice as a control group were gavaged sesame oil solvent in the same manner. The changes in body mass and number of corpora lutea of the pregnant mice were observed; and the body mass and anogenital distance （AGD） of their F1 foetuses were recorded, too. The concentrations of estradiol （ E2 ） and progesterone （P） in sera of the pregnant mice were determined using radioimmunoassay. RESULTS： Reproductive toxicity： with the increase of the administered MXC dose, the nidation quantity decreased, the AGD in the male foetuses exhibited shorter than that in the controls, and the concentrations of E2 and P in sera went up. Significant differences in these indexes were found between the MXC treated groups and the control group （P〈0. 05）. Developmental toxicity： with the increase of the administered MXC dose, the gained weights of pregnant mice reduced, the quantity of live fetuses decreased, and the incidence of adverse pregnancy outcome （fetal death, absorption） went up. The difference among the different-dose MXC groups and the control group were significant difference （P 〈 0.05 ）. CONCLUSION： MXC can disrupt reproductive function, thereby resulting in dysgenesis and maldevelopment in mice.