摘要
目的探讨加味四君子汤治疗脾虚证的机理。方法运用HE染色显示胃组织结构,运用免疫组织化学方法显示下颌下腺EGF含量的变化,运用免疫印迹法显示胃黏膜ERK2含量的变化。结果经过加味四君子汤治疗后,脾虚胃溃疡大鼠下颌下腺颗粒曲管细胞内EGF阳性反映产物的含量减少,胃黏膜磷酸化的ERK2增加,胃溃疡愈合加快;而自然恢复组上述变化不明显。结论加味四君子汤可能通过影响分裂原激活的蛋白激酶信号传递途径而发挥治疗作用。
Objective To study the role of ‘plus sijunzi decoction＇ in the healing of experimental PiXu syndrome combined with gastric ulcer. Methods Using hematoxylin-eosin staining, immunohistochemical technique, and Western-blotting to show the gastric mucosa structure, the phosphorylation level of ERK2 in the gastric mucosa, and the contents of EGF in the granular convoluted tubule （GCT） cells of the submandibular glands respectively. Results After treatment with‘plus sijunzi decoction＇, the phosphorylation level of ERK2 in the gastric mucosa decreased, and the contents of EGF in the GCT cells of the submandibular glands increased in contrast to the control group respectively. The recovery of gastric ulcer was accelerated. However, little change was found in spontaneously recovering group. Conclusion ＇Plus sijunzi decoction＇ accelerates the healing of pi-xu syntrom combined with gastric ulcer by mechanisms involving the increased release of EGF from the submandibular glands and the consequent up-regulation of p-ERK2 in the gastric mucousa.
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
2007年第1期6-11,共6页
Chinese Journal of Histochemistry and Cytochemistry
基金
北京市中医药管理局科技发展基金资助项目(JJ-2001-32)
