功能障碍性子宫出血患者子宫内膜Ⅷ-R Ag和MMP-9的表达

EXPRESSION OF Ⅷ-R AG AND MMP-9 IN THE ENDOMETRIUM OF PATIENTS WITH DYSFUNCTIONAL UTERINE BLEEDING

目的探讨Ⅷ因子相关抗原(Ⅷ-R Ag)和基质金属蛋白酶-9(MMP-9)在功能障碍性子宫出血(DUB)患者子宫内膜的表达。方法采用免疫组织化学方法,对正常妇女与DUB患者的子宫内膜组织中Ⅷ-R Ag和MMP-9进行标记,并应用图像分析技术对其进行定量观察与分析。结果DUB出血区子宫内膜Ⅷ-R Ag表达显著低于其无出血区内膜和正常内膜,出血区子宫内膜MMP-9表达比其无出血部位内膜和正常内膜明显增多。结论DUB患者内膜组织异常出血和脱落与Ⅷ-R Ag和MMP-9异常表达有关。

Objective To investigate the expression of Ⅷ-R Ag and MMP-9 in the endometrium of patients with dysfunctional uterine bleeding （DUB）. Methods Immunohistochemical method was used to study the MMP-9 and Ⅷ-R Ag in the endometrium of twenty cases of normal proliferative phase and twenty cases of DUB. Image analysis was applied to measure the expression of the two proteins. Results The results showed that Ⅷ-R Ag immunoreactivity was significantly lower in bleeding areas of DUB in comparison with the endometrium of its nonbleeding areas and normal proliferative phase. However, the expression of MMP-9 was significantly higher in bleeding areas. Conclusion The results suggest that DUB is associated with disregulation of Ⅷ-R Ag and MMP-9 in the endometrium. It will result in the collapse of angiogenesis and matrix, and lead to the irregular uterine bleeding.